Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jun;69(6):1625–1628. doi: 10.1073/pnas.69.6.1625

Introduction of the 3-Deoxy Group in 3,6-Dideoxyhexoses: A Novel Cofactor Function for Pyridoxamine-5′-Phosphate

Pedro Gonzalez-Porque 1,*, Jack L Strominger 1,†
PMCID: PMC426762  PMID: 4504380

Abstract

A cofactor needed for the reduction of CDP-4-keto-6-deoxy-D-glucose to CDP-4-keto-3,6-dideoxy D-glucose has been detected and purified. Its spectra in neutral, acid, and basic solutions, its behavior on thin-layer chromatography, and other properties led to its identification as pyridoxamine-5′-phosphate. Moreover, authentic pyridoxamine-5′-phosphate substituted for the purified cofactor with about the same specific activity. Other vitamin B6 derivatives did not stimulate the reaction, and inhibition was observed with some of them.

Keywords: vitamin B6, analogues, purified enzymes, polysaccharide components

Full text

PDF
1625

Images in this article

1627Image
on p.1627

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Matsuhashi M., Strominger J. L. Thymidine diphosphate 4-acetamido-2,6-dideoxyhexoses. 3. Purification and properties of thymidine diphosphate 4-keto-6-deoxy-D-glucose transaminase from Escherichia coli strain B. J Biol Chem. 1966 Oct 25;241(20):4738–4744. [PubMed] [Google Scholar]
  3. Matsuhashi S., Matsuhashi M., Strominger J. L. Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexoses. I. Over-all reactions. J Biol Chem. 1966 Sep 25;241(18):4267–4274. [PubMed] [Google Scholar]
  4. Oashi H., Matsuhashi M., Matsuhashi S. Thymidine diphosphate 4-acetamido-4,6-dideoxyhexoses. IV. Purification and properties of thymidine diphosphate 4-keto-6-deoxy-D-glucose transaminase from Pasteurella pseudotuberculosis. J Biol Chem. 1971 Apr 25;246(8):2325–2330. [PubMed] [Google Scholar]
  5. Pape H., Strominger J. L. Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexoses. V. Partial purification of the two protein components required for introduction of the 3-deoxy group. J Biol Chem. 1969 Jul 10;244(13):3598–3604. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES