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. 1972 Jul;69(7):1707–1710. doi: 10.1073/pnas.69.7.1707

Functional Nonequivalence of α and β Hemes in Human Adult Hemoglobin

Ted R Lindstrom 1,2, Chien Ho 1,2,*
PMCID: PMC426783  PMID: 4505648

Abstract

Nuclear magnetic resonance studies of the contact-shifted spectra of heme protons in deoxyhemoglobin A from human adults show conclusively that oxygen binds to the α hemes in preference to the β hemes. The preferential binding is produced in 10% hemoglobin solution at neutral pH by either a 15-fold molar excess of 2,3-diphosphoglycerate or a 5-fold molar excess of inositol hexaphosphate. Preferential binding is not observable in the absence of the organic phosphates. The results indicate that the oxygenation of hemoglobin may be described by a sequential model, or by a concerted model that allows the α hemes to bind ligand first.

Keywords: NMR, preferential ligand binding, organic phosphates, models of cooperative ligand binding

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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