Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Transfusion. 2014 Jul 20;54(12):3186–3197. doi: 10.1111/trf.12755

Figure 3. Phagocytosis in vitro of refrigerator-stored RBCs does not induce significant MCP-1 secretion by J774A.1 cells and primary splenocytes.

Figure 3

J774A.1 cells (left) or primary splenocytes (right) were incubated with PBS, fresh RBCs, or 14-day refrigerator-stored RBCs (“Old”) for one hour in complete medium at 37°C in 5% CO2 at a RBC:macrophage ratio of 50:1. After incubation and subsequent removal of non-ingested RBCs by brief osmotic shock, the phagocytic cells were incubated in incomplete RPMI 1640 medium for 4 hours and secreted MCP-1 was quantified, as described in Materials and Methods. Shown are representative examples of at least 3 experiments.