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. 1972 Jul;69(7):1781–1785. doi: 10.1073/pnas.69.7.1781

DNA Polymerase of Chicken Embryo: Purification and Properties*

Jannis G Stavrianopoulos 1, John D Karkas 1, Erwin Chargaff 1
PMCID: PMC426801  PMID: 4505656

Abstract

The almost complete purification of the previously reported DNA polymerase of chicken embryo is described; in the final isolation step the enrichment of the enzyme, which comprises about 0.01% of the protein extractable from 10-day-old embryos, is more than 9000-fold. The enzyme is a basic protein (pI = 9.15), of molecular weight about 27,000. It exhibits no nuclease activity, requires Mn2+ ion for activity, and may contain Zn. It is very sensitive to oxidation.

Keywords: poly(rA)·poly(dT) template, 9000-fold purified, Mn2+

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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