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. 1972 Jul;69(7):1804–1808. doi: 10.1073/pnas.69.7.1804

Natural-Abundance Carbon-13 Fourier-Transform Nuclear Magnetic Resonance Spectra and Spin Lattice Relaxation Times of Unfractionated Yeast Transfer-RNA*

Richard A Komoroski 1, Adam Allerhand 1,†
PMCID: PMC426806  PMID: 4558659

Abstract

High-resolution Fourier-transform nuclear magnetic resonance at 15.18 MHz was used to observe the proton-decoupled natural-abundance 13C spectra of aqueous unfractionated tRNA from baker's yeast in the presence of Mg+2 (8 ions per tRNA molecule), as a function of temperature in the range of 27-82°C. The spectrum of thermally denatured tRNA at 82°C showed numerous sharp resonances, which were assigned to specific types of carbon atoms by comparison with the 13C spectra of mononucleotides. Only the resonance of carbon 4′ of the ribose rings was appreciably shifted (by about 1.5 ppm upfield) with its average position in the mononucleotides. This effect was also seen in the spectrum of poly(A). In the spectrum of folded tRNA (52°C), carbon 4′ was further shifted upfield by about 1.5 ppm, and carbons 2′ and 3′, which yielded a single resonance at 82°C, now showed two partly resolved peaks.

The variation of linewidths with temperature (200 mg/ml of tRNA) was gradual in the range 27-82°C, and did not reflect the expected unfolding behavior of tRNA. Moreover, dilution to 80 mg/ml at 27°C had the same effect as an increase in temperature to about 45°C. The line-width changes below 60° were ascribed to tRNA aggregation. In contrast to the behavior of the linewidths, the 13C spin-lattice relaxation times (T1) of individual ribose carbon atoms, measured by means of partially relaxed Fourier-transform spectra were practically independent of temperature up to about 60°C, and increased rapidly at higher temperatures. The T1 values indicated that the backbone of thermally denatured tRNA is undergoing rapid segmental motion, with an effective correlation time of (2.6 ± 0.5) × 10-10 sec. The T1 values of folded tRNA yielded no evidence of segmental motion. The correlation time for overall rotational reorientation is about (3 ± 1) × 10-8 sec in the range 35-54°C.

Within experimental error, the T1 values of methine carbons of the bases were equal to those of the methine carbons on the backbone at all temperatures. Only an upper limit to the rate of internal rotation of the bases could be established.

Keywords: mg++, temperature, concentration, linewidths, aggregation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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