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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jul;69(7):1843–1847. doi: 10.1073/pnas.69.7.1843

Initiation Factor IF-3-Dependent Binding of Escherichia coli Ribosomes and N-Formylmethionine Transfer-RNA to Rabbit Globin Messenger

Markus Noll *, Hans Noll *, Jerry B Lingrel
PMCID: PMC426815  PMID: 4558660

Abstract

An initiation complex has been formed in high yields from E. coli ribosomes, 9S messenger RNA for rabbit hemoglobin, and N-formylmethionine-tRNA. Initiation factor IF-3 is required for the binding and puromycin is required for the release of fMet. Valyl-tRNA fails to bind to the second codon, whereas a mixture of 15 aminoacyl-tRNAs promotes incorporation. Together with quantitative data, the findings suggest that IF-3 directs the ribosomes to an AUG codon on one of the two globin messengers, at a site that is different from the normal starting point for globin synthesis.

Keywords: puromycin, AUG codon, valyl-tRNA binding, protein synthesis, hemoglobin

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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