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. 1972 Jul;69(7):1877–1882. doi: 10.1073/pnas.69.7.1877

Polyadenylic Acid at the 3′-Terminus of Poliovirus RNA*

Yoshiaki Yogo 1, Eckard Wimmer 1
PMCID: PMC426823  PMID: 4340162

Abstract

Poliovirus RNA that has been derivatized at the 3′-end with NaIO4-NaB3H4 yields, after hydrolysis with alkali or RNase T2, predominantly labeled residues of modified adenosine; no labeled nucleoside derivative is produced by digestion with RNase A or RNase T1. The 3′-terminal bases of the RNA are, therefore,...ApAOH. Hydrolyzates of poliovirus [32P]RNA, after exhaustive digestion with RNase T1 or RNase A, contain, besides internal oligonucleotides, polynucleotides resistant to further action of ribonucleases T1 and A, respectively; these polynucleotides were isolated by membrane-filter binding or ion-exchange chromatography. The sequence of the T1-resistant polynucleotide was determined to be (Ap)nAOH, that of the RNase A-resistant polynucleotide was GpGp(Ap)nAOH. The chain length (n) of the polyadenylic acid, as analyzed by different methods, averages 89 nucleotides. Gel electrophoresis revealed heterogeneity of the size of poly(A). Poliovirus RNA, when labeled in vitro at the 3′-end, contains [3′-3H]poly(A); when labeled in vivo with [3H]A, it contains [3H](Ap)nAOH. The data establish that... YpGpGp(Ap)[unk]AOH is the 3′-terminal sequence of poliovirus RNA, Type 1 (Mahoney). Since this mammalian virus reproduces in the cell cytoplasm, these observations may modify prior interpretations of the function of polyadenylate ends on messenger RNAs.

Keywords: chemical labeling, enzymatic fragmentation, Millipore-filter binding, column chromatography, gel electrophoresis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong J. A., Edmonds M., Nakazato H., Phillips B. A., Vaughn M. H. Polyadenylic acid sequences in the virion RNA of poliovirus and Eastern Equine Encephalitis virus. Science. 1972 May 5;176(4034):526–528. doi: 10.1126/science.176.4034.526. [DOI] [PubMed] [Google Scholar]
  2. BEERS R. F., Jr Hydrolysis of polyadenylic acid by pancreatic ribonuclease. J Biol Chem. 1960 Aug;235:2393–2398. [PubMed] [Google Scholar]
  3. Banerjee A. K., Ward R., Shatkin A. J. Cytosine at the 3'-termini of reovirus genome and in vitro mRNA. Nat New Biol. 1971 Jul 28;232(30):114–115. doi: 10.1038/newbio232114a0. [DOI] [PubMed] [Google Scholar]
  4. Bellamy A. R., Joklik W. K. Studies on the A-rich RNA of reovirus. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1389–1395. doi: 10.1073/pnas.58.4.1389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burr H., Lingrel J. B. Poly A sequences at the 3' termini of rabbit globin mRNAs. Nat New Biol. 1971 Sep 8;233(36):41–43. doi: 10.1038/newbio233041a0. [DOI] [PubMed] [Google Scholar]
  6. Cole C. N., Smoler D., Wimmer E., Baltimore D. Defective interfering particles of poliovirus. I. Isolation and physical properties. J Virol. 1971 Apr;7(4):478–485. doi: 10.1128/jvi.7.4.478-485.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cory S., Adams J. M., Spahr P. F., Rensing U. Sequence of 51 nucleotides at the 3'-end of R17 bacteriophage RNA. J Mol Biol. 1972 Jan 14;63(1):41–56. doi: 10.1016/0022-2836(72)90520-7. [DOI] [PubMed] [Google Scholar]
  8. Darnell J. E., Philipson L., Wall R., Adesnik M. Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA. Science. 1971 Oct 29;174(4008):507–510. doi: 10.1126/science.174.4008.507. [DOI] [PubMed] [Google Scholar]
  9. Darnell J. E., Wall R., Tushinski R. J. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321–1325. doi: 10.1073/pnas.68.6.1321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. De Wachter R., Fiers W. Studies on the bacteriophage MS2. IV. The 3'-OH terminal undecanucleotide sequence of the viral RNA chain. J Mol Biol. 1967 Dec 28;30(3):507–527. [PubMed] [Google Scholar]
  11. Edmonds M., Caramela M. G. The isolation and characterization of adenosine monophosphate-rich polynucleotides synthesized by Ehrlich ascites cells. J Biol Chem. 1969 Mar 10;244(5):1314–1324. [PubMed] [Google Scholar]
  12. Edmonds M., Vaughan M. H., Jr, Nakazato H. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336–1340. doi: 10.1073/pnas.68.6.1336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Erikson R. L., Erikson E., Walker T. A. The identification of the 3'-hydroxyl nucleoside terminus of avian myeloblastosis virus RNA. Virology. 1971 Aug;45(2):527–528. doi: 10.1016/0042-6822(71)90354-0. [DOI] [PubMed] [Google Scholar]
  14. Gilham P. T., Rosenberg M. The isolation of 3'-terminal polynucleotides from RNA molecules. Biochim Biophys Acta. 1971 Aug 26;246(2):337–340. doi: 10.1016/0005-2787(71)90143-2. [DOI] [PubMed] [Google Scholar]
  15. Glitz D. G., Bradley A., Fraenkel-Contrat H. Nucleotide sequences at the 5'-linked ends of viral ribonucleic acids. Biochim Biophys Acta. 1968 Jun 18;161(1):1–12. doi: 10.1016/0005-2787(68)90288-8. [DOI] [PubMed] [Google Scholar]
  16. Goodman H. M., Billeter M. A., Hindley J., Weissmann C. The nucleotide sequence at the 5'-terminus of the Q RNA minus trand. Proc Natl Acad Sci U S A. 1970 Oct;67(2):921–928. doi: 10.1073/pnas.67.2.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Granboulan N., Girard M. Molecular weight of poliovirus ribonucleic acid. J Virol. 1969 Oct;4(4):475–479. doi: 10.1128/jvi.4.4.475-479.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hadjivassiliou A., Brawerman G. Polyadenylic acid in the cytoplasm of rat liver. J Mol Biol. 1966 Sep;20(1):1–7. doi: 10.1016/0022-2836(66)90112-4. [DOI] [PubMed] [Google Scholar]
  19. Horst J., Fraenkel-Conrat H., Mandeles S. Terminal heterogeneity at both ends of the satellite tobacco necrosis virus ribonucleic acid. Biochemistry. 1971 Dec 7;10(25):4748–4752. doi: 10.1021/bi00801a022. [DOI] [PubMed] [Google Scholar]
  20. Kamen R. Infectivity of bacteriophage R17 RNA after sequential removal of 3' terminal nucleotides. Nature. 1969 Jan 25;221(5178):321–325. doi: 10.1038/221321a0. [DOI] [PubMed] [Google Scholar]
  21. Katz L., Penman S. The solvent denaturation of double-stranded RNA from poliovirus infected HeLa cells. Biochem Biophys Res Commun. 1966 May 25;23(4):557–560. doi: 10.1016/0006-291x(66)90765-0. [DOI] [PubMed] [Google Scholar]
  22. Lee S. Y., Mendecki J., Brawerman G. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331–1335. doi: 10.1073/pnas.68.6.1331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lewandowski L. J., Content J., Leppla S. H. Characterization of the subunit structure of the ribonucleic acid genome of influenza virus. J Virol. 1971 Nov;8(5):701–707. doi: 10.1128/jvi.8.5.701-707.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Lim L., Canellakis E. S. Adenine-rich polymer associated with rabbit reticulocyte messenger RNA. Nature. 1970 Aug 15;227(5259):710–712. doi: 10.1038/227710a0. [DOI] [PubMed] [Google Scholar]
  25. Loening U. E. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation. Biochem J. 1969 Jun;113(1):131–138. doi: 10.1042/bj1130131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Maruyama H. B., Hatanaka M., Gilden R. V. The 3'-terminal nucleosides of the high molecular weight RNA of C-type viruses. Proc Natl Acad Sci U S A. 1971 Sep;68(9):1999–2001. doi: 10.1073/pnas.68.9.1999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Mendecki J., Lee S. Y., Brawerman G. Characteristics of the polyadenylic acid segment associated with messenger ribonucleic acid in mouse sarcoma 180 ascites cells. Biochemistry. 1972 Feb 29;11(5):792–798. doi: 10.1021/bi00755a018. [DOI] [PubMed] [Google Scholar]
  28. Philipson L., Wall R., Glickman G., Darnell J. E. Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806–2809. doi: 10.1073/pnas.68.11.2806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. RajBhandary U. L. Studies on polynucleotides. LXXVII. The labeling of end groups in polynucleotide chains: the selective modification of diol end groups in ribonucleic acids. J Biol Chem. 1968 Feb 10;243(3):556–564. [PubMed] [Google Scholar]
  30. Rensing U., August J. T. The 3'-terminus and the replication of phage RNA. Nature. 1969 Nov 29;224(5222):853–856. doi: 10.1038/224853a0. [DOI] [PubMed] [Google Scholar]
  31. Stavis R. L., August J. T. The biochemistry of RNA bacteriophage replication. Annu Rev Biochem. 1970;39:527–560. doi: 10.1146/annurev.bi.39.070170.002523. [DOI] [PubMed] [Google Scholar]
  32. Weber H., Weissmann C. The 3'-termini of bacteriophage Q-beta plus and minus strands. J Mol Biol. 1970 Jul 28;51(2):215–224. doi: 10.1016/0022-2836(70)90138-5. [DOI] [PubMed] [Google Scholar]
  33. Weinberg R. A., Penman S. Small molecular weight monodisperse nuclear RNA. J Mol Biol. 1968 Dec;38(3):289–304. doi: 10.1016/0022-2836(68)90387-2. [DOI] [PubMed] [Google Scholar]
  34. Wimmer E., Reichmann M. E. Two 3'-terminal sequences in satellite tobacco necrosis virus RNA. Nature. 1969 Mar 22;221(5186):1122–1126. doi: 10.1038/2211122a0. [DOI] [PubMed] [Google Scholar]

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