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. 1972 Jul;69(7):1929–1933. doi: 10.1073/pnas.69.7.1929

Cell Cycle Changes and the Ability of Cells to Undergo Virus-Induced Fusion

Joan K Stadler 1, Edward A Adelberg 1
PMCID: PMC426834  PMID: 4340165

Abstract

Synchronized cells of the mouse leukemic cell line, L5178Y, were examined for their ability to undergo virus-induced fusion. Fusion capacity (defined as the percentage of the original unicellular population that is induced by virus to participate in a fusion event) was measured in cells that were (i) arrested by colcemid, (ii) released from colcemid block, and (iii) released from thymidine block. The results show that fusion capacity is minimal during G1 phase (less than 10%), and rises to a maximum (69%) in arrested mitosis. Treatment of nonsynchronized cells with trypsin increased their fusion capacity to the level characteristic of cells arrested in mitosis. Colcemid-arrested cells produced twice as many hybrid clones as nonsynchronized cells after they were plated in selective medium.

Keywords: mouse lymphocytes, cell surface, colcemid, hybrids

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bosmann H. B., Winston R. A. Synthesis of glycoprotein, glycolipid, protein, and lipid in synchronized L5178Y cells. J Cell Biol. 1970 Apr;45(1):23–33. doi: 10.1083/jcb.45.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burger M. M. A difference in the architecture of the surface membrane of normal and virally transformed cells. Proc Natl Acad Sci U S A. 1969 Mar;62(3):994–1001. doi: 10.1073/pnas.62.3.994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chu M. Y., Fischer G. A. The incorporation of 3H-cytosine arabinoside and its effect on murine leukemic cells (L5178Y). Biochem Pharmacol. 1968 May;17(5):753–767. doi: 10.1016/0006-2952(68)90012-9. [DOI] [PubMed] [Google Scholar]
  4. FISCHER G. A. Studies of the culture of leukemic cells in vitro. Ann N Y Acad Sci. 1958 Dec 5;76(3):673–680. doi: 10.1111/j.1749-6632.1958.tb54884.x. [DOI] [PubMed] [Google Scholar]
  5. Flamm W. G., Bernheim N. J., Brubaker P. E. Density gradient analysis of newly replicated DNA from synchronized mouse lymphoma cells. Exp Cell Res. 1971 Jan;64(1):97–104. doi: 10.1016/0014-4827(71)90197-2. [DOI] [PubMed] [Google Scholar]
  6. Fox T. O., Sheppard J. R., Burger M. M. Cyclic membrane changes in animal cells: transformed cells permanently display a surface architecture detected in normal cells only during mitosis. Proc Natl Acad Sci U S A. 1971 Jan;68(1):244–247. doi: 10.1073/pnas.68.1.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kao F. T., Puck T. T. Genetics of somatic mammalian cells. IX. Quantitation of mutagenesis by physical and chemical agents. J Cell Physiol. 1969 Dec;74(3):245–258. doi: 10.1002/jcp.1040740305. [DOI] [PubMed] [Google Scholar]
  8. Klebe R. J., Chen T., Ruddle F. H. Controlled production of proliferating somatic cell hybrids. J Cell Biol. 1970 Apr;45(1):74–82. doi: 10.1083/jcb.45.1.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  10. Lucy J. A. The fusion of biological membranes. Nature. 1970 Aug 22;227(5260):815–817. doi: 10.1038/227815a0. [DOI] [PubMed] [Google Scholar]
  11. Makman M. H., Klein M. I. Expression of adenylate cyclase, catecholamine receptor, and cyclic adenosine monophosphate-dependent protein kinase in synchronized culture of Chang's liver cells (S phase-membrane receptors-NaF stimulation). Proc Natl Acad Sci U S A. 1972 Feb;69(2):456–458. doi: 10.1073/pnas.69.2.456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Poste G. Virus-induced polykaryocytosis and the mechanism of cell fusion. Adv Virus Res. 1970;16:303–356. doi: 10.1016/s0065-3527(08)60026-3. [DOI] [PubMed] [Google Scholar]
  13. WELSHONS W. J., GIBSON B. H., SCANDLYN B. J. Slide processing for the examination of male mammalian meiotic chromosomes. Stain Technol. 1962 Jan;37:1–5. doi: 10.3109/10520296209114560. [DOI] [PubMed] [Google Scholar]

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