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. 1972 Jul;69(7):1934–1938. doi: 10.1073/pnas.69.7.1934

Demonstration That Antigen-Binding Cells Are Precursors of Antibody-Producing Cells After Purification with a Fluorescence-Activated Cell Sorter

M H Julius 1, T Masuda 1, L A Herzenberg 1,*
PMCID: PMC426835  PMID: 4114858

Abstract

We have obtained viable and functional populations of antigen-binding cells enriched up to 500-fold from primed spleen-cell suspensions by fluorescent labeling and by a new electronic cell sorter that sorts viable cells according to fluorescence. Concomitantly, populations largely depleted of antigen-binding cells were obtained. While neither population alone is capable of a full adoptive secondary response when injected into irradiated recipients, a reconstituted mixture restores the full response of the unfractionated spleen cells. Admixture of sources of unprimed thymus-derived cells (T-cells) with the purified antigen-binding cells (B-cells) restores much of the full response.

Keywords: keyhole-limpet hemocyanin; human-serum albumin; adoptive secondary response; lymphocyte receptors; thymus-derived, bone marrow-derived cells

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1934

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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