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. 1972 Jul;69(7):1967–1971. doi: 10.1073/pnas.69.7.1967

Purification and Properties of Biologically Active Messenger RNA for a Myeloma Light Chain

D Swan 1, H Aviv 1, P Leder 1
PMCID: PMC426842  PMID: 4505674

Abstract

A cell-free system derived from Krebs II ascites tumor has been used to assay biologically active mRNA for myeloma (MOPC-41) light chain during its purification by oligothymidylate-cellulose chromatography and sucrose gradient centrifugation. The purified mRNA directs the synthesis of a product that yields tryptic peptides corresponding to those derived from authentic myeloma protein and that forms a specific immunoprecipitate with antibody directed against the MOPC-41 protein. The fact that the light-chain mRNA anneals to oligothymidylic acid-cellulose suggests that it, like several other eukaryotic mRNAs, contains a region rich in adenylic acid residues. The most active fractions of light-chain mRNA, representing about 0.1% of the RNA originally extracted from membrane-bound myeloma polysomes, sediment as a discrete peak with an s20,w of about 13, roughly corresponding to an RNA molecule containing 850 bases. The results suggest that the light-chain mRNA is monocistronic and that it contains about 200 more bases than would be necessary to encode the variable and constant regions of a single light-chain molecule.

Keywords: poly(A)-mRNA, oligo d(T)-cellulose, monocistronic 13S mRNA

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Selected References

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