Abstract
An experimental procedure is detailed that permits the detection of 70S RNA-directed DNA synthesis in mouse mammary carcinomas. The DNA synthesized is complementary to the RNA of the mouse mammary tumor virus by molecular hybridization, thus, completing the proof that an RNA-instructed DNA polymerase has been identified. Further, RNA-instructed DNA polymerase and its 70S RNA template are physically associated in a particle that has a density characteristic of oncornaviruses. These experiments now provide the technology required to perform similar examinations of human neoplasias.
Keywords: 70S RNA, mouse mammary carcinoma, oncornaviruses
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Axel R., Schlom J., Spiegelman S. Evidence for translation of viral-specific RNA in cells of a mouse mammary carcinoma. Proc Natl Acad Sci U S A. 1972 Mar;69(3):535–538. doi: 10.1073/pnas.69.3.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Axel R., Schlom J., Spiegelman S. Presence in human breast cancer of RNA homologous to mouse mammary tumour virus RNA. Nature. 1972 Jan 7;235(5332):32–36. doi: 10.1038/235032a0. [DOI] [PubMed] [Google Scholar]
- Hehlmann R., Kufe D., Spiegelman S. RNA in human leukemic cells related to the RNA of a mouse leukemia virus (leukocytes-RNA-DNA hybridization-rauscher virus-polysomal RNA). Proc Natl Acad Sci U S A. 1972 Feb;69(2):435–439. doi: 10.1073/pnas.69.2.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hehlmann R., Kufe D., Spiegelman S. Viral-related RNA in Hodgkins' disease and other human lymphomas. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1727–1731. doi: 10.1073/pnas.69.7.1727. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kufe D., Hehlmann R., Spiegelman S. Human sarcomas contain RNA related to the RNA of a mouse leukemia virus. Science. 1972 Jan 14;175(4018):182–185. doi: 10.1126/science.175.4018.182. [DOI] [PubMed] [Google Scholar]
- Schlom J., Spiegelman S., Moore D. H. Detection of high-molecular-weight RNA in particles from human milk. Science. 1972 Feb 4;175(4021):542–544. doi: 10.1126/science.175.4021.542. [DOI] [PubMed] [Google Scholar]
- Schlom J., Spiegelman S. Simultaneous detection of reverse transcriptase and high molecular weight RNA unique to oncogenic RNA viruses. Science. 1971 Nov 19;174(4011):840–843. doi: 10.1126/science.174.4011.840. [DOI] [PubMed] [Google Scholar]
- Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Travnicek M., Watson K. Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses. Nature. 1970 Aug 8;227(5258):563–567. doi: 10.1038/227563a0. [DOI] [PubMed] [Google Scholar]