Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Aug;69(8):2095–2099. doi: 10.1073/pnas.69.8.2095

Translation of Messenger RNA for Histones from HeLa Cells by a Cell-Free Extract from Mouse Ascites Tumor

M Jacobs-Lorena *, C Baglioni *, T W Borun
PMCID: PMC426876  PMID: 4506079

Abstract

7-9S RNA has been isolated from polyribosomes of HeLa S-3 cells in S phase. These RNA species direct the synthesis in vitro of all five classes of human histones when they are added to a protein-synthesizing system derived from mouse ascites tumor cells. The histones synthesized in vitro have been identified by two kinds of acrylamide gel electrophoresis, and by paper electrophoresis of tryptic digests. By use of the ratio of tyrosine to tryptophan incorporation as an index of contamination, it was determined that less than 10% of the 7-9S RNA is translated into proteins other than histones.

Keywords: 7-9S RNA, histones, protein synthesis, acrylamide gel electrophoresis

Full text

PDF
2095

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baglioni C., La Via M., Ventruto V. A study of immunoglobulin structure. I. The fingerprinting of aminoethylated Bence-Jones proteins. Biochim Biophys Acta. 1965 Dec 16;111(2):479–484. doi: 10.1016/0304-4165(65)90057-7. [DOI] [PubMed] [Google Scholar]
  2. Berns A. J.M., De Abreu R. A., Van Kraaikamp M., Benedetti E. L., Bloemendal H. Synthesis of lens protein in vitro V. Isolation of messenger-like RNA from lens by high resolution zonal centrifugation. FEBS Lett. 1971 Oct 15;18(1):159–163. doi: 10.1016/0014-5793(71)80434-9. [DOI] [PubMed] [Google Scholar]
  3. Bishop J. O., Pemberton R., Baglioni C. Reiteration frequency of haemoglobin genes in the duck. Nat New Biol. 1972 Feb 23;235(60):231–234. doi: 10.1038/newbio235231a0. [DOI] [PubMed] [Google Scholar]
  4. Borun T. W., Scharff M. D., Robbins E. Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1977–1983. doi: 10.1073/pnas.58.5.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Braunitzer G., Best J. S., Flamm U., Schrank B. Zur Phylogenie des Hämoglobins: Untersuchungen am Hämoglobin des Kaninchens (Caniculus) Hoppe Seylers Z Physiol Chem. 1966;347(4):207–211. [PubMed] [Google Scholar]
  6. Gallwitz D., Mueller G. C. Histone synthesis in vitro by cytoplasmic microsomes from HeLa cells. Science. 1969 Mar 21;163(3873):1351–1353. doi: 10.1126/science.163.3873.1351. [DOI] [PubMed] [Google Scholar]
  7. Gallwitz D., Mueller G. C. Histone synthesis in vitro on HeLa cell microsomes. The nature of the coupling to deoxyribonucleic acid synthesis. J Biol Chem. 1969 Nov 10;244(21):5947–5952. [PubMed] [Google Scholar]
  8. Housman D., Pemberton R., Taber R. Synthesis of and chains of rabbit hemoglobin in a cell-free extract from Krebs II ascites cells. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2716–2719. doi: 10.1073/pnas.68.11.2716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. JOHNS E. W., BUTLER J. A. Further fractionations of histones from calf thymus. Biochem J. 1962 Jan;82:15–18. doi: 10.1042/bj0820015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jacobs-Lorena M., Baglioni C. Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1425–1428. doi: 10.1073/pnas.69.6.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kedes L. H., Birnstiel M. L. Reiteration and clustering of DNA sequences complementary to histone messenger RNA. Nat New Biol. 1971 Apr 7;230(14):165–169. doi: 10.1038/newbio230165a0. [DOI] [PubMed] [Google Scholar]
  12. Kedes L. H., Gross P. R. Identification in cleaving embryos of three RNA species serving as templates for the synthesis of nuclear proteins. Nature. 1969 Sep 27;223(5213):1335–1339. doi: 10.1038/2231335a0. [DOI] [PubMed] [Google Scholar]
  13. Kedes L. H., Gross P. R. Synthesis and function of messenger RNA during early embryonic development. J Mol Biol. 1969 Jun 28;42(3):559–575. doi: 10.1016/0022-2836(69)90243-5. [DOI] [PubMed] [Google Scholar]
  14. Lockard R. E., Lingrel J. B. The synthesis of mouse hemoglobin beta-chains in a rabbit reticulocyte cell-free system programmed with mouse reticulocyte 9S RNA. Biochem Biophys Res Commun. 1969 Oct 8;37(2):204–212. doi: 10.1016/0006-291x(69)90720-7. [DOI] [PubMed] [Google Scholar]
  15. Maizel J. V., Jr Acrylamide-gel electrophorograms by mechanical fractionation: radioactive adenovirus proteins. Science. 1966 Feb 25;151(3713):988–990. doi: 10.1126/science.151.3713.988. [DOI] [PubMed] [Google Scholar]
  16. Mathews M. B., Korner A. The inhibitory action of a mammalian viral RNA on the initiation of protein synthesis in a reticulocyte cell-free system. Eur J Biochem. 1970 Dec;17(2):339–343. doi: 10.1111/j.1432-1033.1970.tb01171.x. [DOI] [PubMed] [Google Scholar]
  17. Moriyama Y., Hodnett J. L., Prestayko A. W., Busch H. Studies on the nuclear 4 to 7 s RNA of the Novikoff hepatoma. J Mol Biol. 1969 Jan;39(2):335–349. doi: 10.1016/0022-2836(69)90321-0. [DOI] [PubMed] [Google Scholar]
  18. NISHIMURA S., NOVELLI G. D. AMINO ACID ACCEPTOR ACTIVITY OF ENZYMICALLY ALTERED SOLUBLE RNA FROM ESCHERICHIA COLI. Biochim Biophys Acta. 1964 Apr 27;80:574–586. doi: 10.1016/0926-6550(64)90302-0. [DOI] [PubMed] [Google Scholar]
  19. Panyim S., Chalkley R. High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969 Mar;130(1):337–346. doi: 10.1016/0003-9861(69)90042-3. [DOI] [PubMed] [Google Scholar]
  20. Robbins E., Borun T. W. The cytoplasmic synthesis of histones in hela cells and its temporal relationship to DNA replication. Proc Natl Acad Sci U S A. 1967 Feb;57(2):409–416. doi: 10.1073/pnas.57.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  22. Schodhetman G., Perry R. P. Early appearance of histone messenger RNA in polyribosomes of cultured L cells. J Mol Biol. 1972 Feb 14;63(3):591–596. doi: 10.1016/0022-2836(72)90450-0. [DOI] [PubMed] [Google Scholar]
  23. Stein G. S., Borun T. W. The synthesis of acidic chromosomal proteins during the cell cycle of HeLa S-3 cells. I. The accelerated accumulation of acidic residual nuclear protein before the initiation of DNA replication. J Cell Biol. 1972 Feb;52(2):292–307. doi: 10.1083/jcb.52.2.292. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES