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. 1972 Aug;69(8):2184–2188. doi: 10.1073/pnas.69.8.2184

Hemoglobin Switching in Sheep and Goats: Change in Functional Globin Messenger RNA in Reticulocytes and Bone Marrow Cells

Arthur W Nienhuis 1, W French Anderson 1
PMCID: PMC426896  PMID: 4506088

Abstract

Anemia causes a change in the type of circulating hemoglobin in goats and certain sheep: HbA (α2β2A) is replaced by HbC (α2β2C). We have isolated globin mRNA from erythroid cells of anemic and nonanemic animals to investigate the mechanism whereby anemia causes this switch. To study several stages in transition from βA to βC synthesis, active globin mRNA was isolated from bone marrow cells, as well as from reticulocytes. By assaying these globin mRNAs in a rabbit reticulocyte cell-free system, we have demonstrated that the switch from βA to βC globin synthesis is mediated via a change in functional globin mRNA.

Keywords: cell culture, β chains, mRNA isolation, anemia, erythroid

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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