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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Aug;69(8):2224–2228. doi: 10.1073/pnas.69.8.2224

Chromatin Structure and the Cell Cycle

Thoru Pederson 1
PMCID: PMC426905  PMID: 4626402

Abstract

Pancreatic DNase I is used to probe the structure of chromatin isolated from synchronized HeLa cells. The degree to which DNA in chromatin is protected from DNase attack varies during the G1, S, and G2 phases of the cell cycle. In addition, the DNase sensitivity of chromatin from contact-inhibited African green monkey kidney cells differs from that of actively dividing, subconfluent cultures. These cell cycle-dependent chromatin changes were observed consistently at all enzyme concentrations (5000-fold range) and incubation times (15 min-2 hr) tested. The results indicate that the degree of complexing between DNA and chromosomal proteins changes during interphase, and they suggest that the chromosome coiling cycle of visible mitosis may extend in more subtle form over the entire cell cycle.

Keywords: DNase I, synchronized HeLa cells, monkey-kidney cells, contact inhibition

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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