Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Aug;69(8):2233–2236. doi: 10.1073/pnas.69.8.2233

In Vitro Synthesis of a Regulator of Mammalian Gene Expression

Harvey R Colten 1,2
PMCID: PMC426907  PMID: 4506091

Abstract

Peritoneal cells isolated from guinea pigs homozygous for a deficiency of the fourth component of complement (C4), produce in vitro a factor that induces the synthesis and secretion of functionally active human C4 by a human cell. This factor appeared to switch on production of C4 in the responsive cell without affecting total protein synthesis. The regulator factor is not species specific, inasmuch as guinea pig regulator affected a corresponding human gene function. Both synthesis of the factor and the response to it were inhibited by actinomycin D. The amount of regulator recovered from genetically deficient cells was about 5-10 times that recovered from normal guinea pig cells.

Keywords: serum complement, genetic deficiency, protein biosynthesis

Full text

PDF
2233

Images in this article

2234Image
on p.2234

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alper C. A., Rosen F. S. Genetic aspects of the complement system. Adv Immunol. 1971;14:251–290. doi: 10.1016/s0065-2776(08)60286-2. [DOI] [PubMed] [Google Scholar]
  2. Borsos T., Rapp H. J. Immune hemolysis: a simplified method for the preparation of EAC'4 with guinea pig or with human complement. J Immunol. 1967 Aug;99(2):263–268. [PubMed] [Google Scholar]
  3. Colten H. R., Frank M. M. Biosynthesis of the second (C2) and fourth (C4) components of complement in vitro by tissues isolated from guinea-pigs with genetically determined C4 deficiency. Immunology. 1972 Jun;22(6):991–999. [PMC free article] [PubMed] [Google Scholar]
  4. Colten H. R., Parkman R. Biosynthesis of C4 (fourth component of complement) by hybrids of C4-deficient guinea pig cells and HeLa cells. Science. 1972 Jun 2;176(4038):1029–1031. doi: 10.1126/science.176.4038.1029. [DOI] [PubMed] [Google Scholar]
  5. Davidson R. L., Benda P. Regulation of specific functions of glial cells in somatic hybrids. II. Control of inducibility of glycerol-3-phosphate dehydrogenase. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1870–1877. doi: 10.1073/pnas.67.4.1870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ellman L., Green I., Frank M. Genetically controlled total deficiency of the fourth component of complement in the guinea pig. Science. 1970 Oct 2;170(3953):74–75. doi: 10.1126/science.170.3953.74. [DOI] [PubMed] [Google Scholar]
  7. Fougère C., Ruiz F., Ephrussi B. Gene dosage dependence of pigment synthesis in melanoma x fibroblast hybrids (hamster cells-mouse fibroblast-DOPA-oxidase-irradiation). Proc Natl Acad Sci U S A. 1972 Feb;69(2):330–334. doi: 10.1073/pnas.69.2.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frank M. M., May J., Gaither T., Ellman L. In vitro studies of complement function in sera of C4-deficient guinea pigs. J Exp Med. 1971 Jul 1;134(1):176–187. doi: 10.1084/jem.134.1.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Opferkuch W., Rapp H. J., Colten H. R., Borsos T. Immune hemolysis and the functional properties of the second (C2) and fourth (C4) components of complement. 3. The hemolytic efficiency of human and guinea pig C2 and C4. J Immunol. 1971 Apr;106(4):927–931. [PubMed] [Google Scholar]
  10. Peterson J. A., Weiss M. C. Expression of differentiated functions in hepatoma cell hybrids: induction of mouse albumin production in rat hepatoma-mouse fibroblast hybrids. Proc Natl Acad Sci U S A. 1972 Mar;69(3):571–575. doi: 10.1073/pnas.69.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES