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. 1972 Aug;69(8):2237–2240. doi: 10.1073/pnas.69.8.2237

Calcium-Dependent Norepinephrine Release from Presynaptic Nerve Endings In Vitro

Mordecai P Blaustein 1,2,3, Eugene M Johnson Jr 1,2,3, Philip Needleman 1,2,3
PMCID: PMC426908  PMID: 4506092

Abstract

Potassium concentrations above 15-20 mM in the medium, or addition of 75 μM veratridine to medium that contains 5 mM K, stimulate calcium accumulation and Ca-dependent norepinephrine release by presynaptic nerve terminals (synaptosomes) incubated in vitro. The effects of veratridine, but not of K, are blocked by 0.2 μM tetrodotoxin. This association between Ca entry and norepinephrine release is consistent with the Calcium Hypothesis of transmitter release. These results add further evidence that synaptosomes may retain many functional properties of intact presynaptic endings.

Keywords: veratridine, K+, tetrodotoxin, synaptosomes, rat, pargyline

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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