Abstract
Three micromethods are described for the assay of enzymes or products of the heme biosynthetic pathway in blood. A fluorometric method for the assay of protoporphyrin may be applied to the rapid screening of children for chronic lead poisoning. A colorimetric assay for δ-aminolevulinic-acid dehydratase may be applied to the detection of acute and chronic lead poisoning. A fluorometric assay for porphyrin formation from prophobilinogen is also described.
Keywords: lead poisoning, iron deficiency, uroporphyrin, coproporphyrin, protoporphyrin
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHISOLM J. J., Jr V/. J Pediatr. 1964 Feb;64:174–187. doi: 10.1016/s0022-3476(64)80261-4. [DOI] [PubMed] [Google Scholar]
- Delves H. T. A micro-sampling method for the rapid determination of lead in blood by atomic-absorption spectrophotometry. Analyst. 1970 May;95(130):431–438. doi: 10.1039/an9709500431. [DOI] [PubMed] [Google Scholar]
- MAUZERALL D., GRANICK S. The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine. J Biol Chem. 1956 Mar;219(1):435–446. [PubMed] [Google Scholar]
- Meyer U. A., Strand L. J., Doss M., Rees A. C., Marver H. S. Intermittent acute porphyria--demonstration of a genetic defect in porphobilinogen metabolism. N Engl J Med. 1972 Jun 15;286(24):1277–1282. doi: 10.1056/NEJM197206152862401. [DOI] [PubMed] [Google Scholar]
- Nakao K., Sassa S., Wada O., Takaku F. Enzymatic studies on erythroid differentiation and proliferation. Ann N Y Acad Sci. 1968 Mar 29;149(1):224–228. doi: 10.1111/j.1749-6632.1968.tb15155.x. [DOI] [PubMed] [Google Scholar]
- Rimington C. Spectral-absorption coefficients of some porphyrins in the Soret-band region. Biochem J. 1960 Jun;75(3):620–623. doi: 10.1042/bj0750620. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATSON C. J. Porphyria. Adv Intern Med. 1954;6:235–299. [PubMed] [Google Scholar]
- Weissberg J. B., Lipschutz F., Oski F. A. Delta-aminolevulinic acid dehydratase activity in circulating blood cells. A sensitive laboratory test for the detection of childhood lead poisoning. N Engl J Med. 1971 Mar 18;284(11):565–569. doi: 10.1056/NEJM197103182841101. [DOI] [PubMed] [Google Scholar]