Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Sep;69(9):2381–2385. doi: 10.1073/pnas.69.9.2381

Assays for Porphyrins, δ-Aminolevulinic-Acid Dehydratase, and Porphyrinogen Synthetase in Microliter Samples of Whole Blood: Applications to Metabolic Defects Involving the Heme Pathway

S Granick 1, S Sassa 1, J L Granick *, R D Levere *, A Kappas 1
PMCID: PMC426945  PMID: 4506758

Abstract

Three micromethods are described for the assay of enzymes or products of the heme biosynthetic pathway in blood. A fluorometric method for the assay of protoporphyrin may be applied to the rapid screening of children for chronic lead poisoning. A colorimetric assay for δ-aminolevulinic-acid dehydratase may be applied to the detection of acute and chronic lead poisoning. A fluorometric assay for porphyrin formation from prophobilinogen is also described.

Keywords: lead poisoning, iron deficiency, uroporphyrin, coproporphyrin, protoporphyrin

Full text

PDF
2381

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHISOLM J. J., Jr V/. J Pediatr. 1964 Feb;64:174–187. doi: 10.1016/s0022-3476(64)80261-4. [DOI] [PubMed] [Google Scholar]
  2. Delves H. T. A micro-sampling method for the rapid determination of lead in blood by atomic-absorption spectrophotometry. Analyst. 1970 May;95(130):431–438. doi: 10.1039/an9709500431. [DOI] [PubMed] [Google Scholar]
  3. MAUZERALL D., GRANICK S. The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine. J Biol Chem. 1956 Mar;219(1):435–446. [PubMed] [Google Scholar]
  4. Meyer U. A., Strand L. J., Doss M., Rees A. C., Marver H. S. Intermittent acute porphyria--demonstration of a genetic defect in porphobilinogen metabolism. N Engl J Med. 1972 Jun 15;286(24):1277–1282. doi: 10.1056/NEJM197206152862401. [DOI] [PubMed] [Google Scholar]
  5. Nakao K., Sassa S., Wada O., Takaku F. Enzymatic studies on erythroid differentiation and proliferation. Ann N Y Acad Sci. 1968 Mar 29;149(1):224–228. doi: 10.1111/j.1749-6632.1968.tb15155.x. [DOI] [PubMed] [Google Scholar]
  6. Rimington C. Spectral-absorption coefficients of some porphyrins in the Soret-band region. Biochem J. 1960 Jun;75(3):620–623. doi: 10.1042/bj0750620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. WATSON C. J. Porphyria. Adv Intern Med. 1954;6:235–299. [PubMed] [Google Scholar]
  8. Weissberg J. B., Lipschutz F., Oski F. A. Delta-aminolevulinic acid dehydratase activity in circulating blood cells. A sensitive laboratory test for the detection of childhood lead poisoning. N Engl J Med. 1971 Mar 18;284(11):565–569. doi: 10.1056/NEJM197103182841101. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES