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. 1972 Sep;69(9):2456–2459. doi: 10.1073/pnas.69.9.2456

Antigen Cap Formation in Cultured Fibroblasts: A Reflection of Membrane Fluidity and of Cell Motility

Michael Edidin 1, Arthur Weiss 1
PMCID: PMC426964  PMID: 4560687

Abstract

Reaction of antibody to surface Histocompatibility-2 antigens of cultured mouse fibroblasts causes aggregation of the cellular antigens into caps; these appear as areas of high antigen concentration localized away from the cell processes. Cap formation is inhibited when ATP generation by cells is interrupted, when the temperature is lowered, by addition of cycloheximide—especially over long time periods—and by colcemid. The observations are consistent with a role of the locomotor system of cells in the collection of small antigen-antibody aggregates into a large localized cap. Together with observations on the absence of caps in epithelial cells, these findings suggest that only cells, such as fibroblasts and lymphocytes, that bear leading ruffled membranes are capable of driving antigenantibody aggregates to form caps.

Keywords: histocompatibility-2, colcemid, fluorescent antibody

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abercrombie M., Heaysman J. E., Pegrum S. M. The locomotion of fibroblasts in culture. 3. Movements of particles on the dorsal surface of the leading lamella. Exp Cell Res. 1970 Oct;62(2):389–398. doi: 10.1016/0014-4827(70)90570-7. [DOI] [PubMed] [Google Scholar]
  2. Abercrombie M., Heaysman J. E., Pegrum S. M. The locomotion of fibroblasts in culture. II. "RRuffling". Exp Cell Res. 1970 Jun;60(3):437–444. doi: 10.1016/0014-4827(70)90537-9. [DOI] [PubMed] [Google Scholar]
  3. Bhisey A. N., Freed J. J. Ameboid movement induced in cultured macrophages by colchicine or vinblastine. Exp Cell Res. 1971 Feb;64(2):419–429. doi: 10.1016/0014-4827(71)90096-6. [DOI] [PubMed] [Google Scholar]
  4. Cikes M., Friberg S., Jr Expression of H-2 and Moloney leukemia virus-determined cell-surface antigens in synchronized cultures of a mouse cell line. Proc Natl Acad Sci U S A. 1971 Mar;68(3):566–569. doi: 10.1073/pnas.68.3.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cone R. A. Rotational diffusion of rhodopsin in the visual receptor membrane. Nat New Biol. 1972 Mar 15;236(63):39–43. doi: 10.1038/newbio236039a0. [DOI] [PubMed] [Google Scholar]
  6. Domnina L. V., Ivanova O. Y., Margolis L. B., Olshevskaja L. V., Rovensky Y. A., Vasiliev J. M., Gelfand I. M. Defective formation of the lamellar cytoplasm by neoplastic fibroblasts (L cells-transformed cells-cell attachment-contact inhibition-scanning electron microscopy-microcinematography). Proc Natl Acad Sci U S A. 1972 Jan;69(1):248–252. doi: 10.1073/pnas.69.1.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Edidin M. A rapid, quantitative fluorescence assay for cell damage by cytotoxic antibodies. J Immunol. 1970 May;104(5):1303–1306. [PubMed] [Google Scholar]
  8. Frye L. D., Edidin M. The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. J Cell Sci. 1970 Sep;7(2):319–335. doi: 10.1242/jcs.7.2.319. [DOI] [PubMed] [Google Scholar]
  9. Gail M. H., Boone C. W. Effect of colcemid on fibroblast motility. Exp Cell Res. 1971 Mar;65(1):221–227. doi: 10.1016/s0014-4827(71)80070-8. [DOI] [PubMed] [Google Scholar]
  10. McFarland W., Heilman D. H., Moorhead J. F. Functional anatomy of the lymphocyte in immunological reactions in vitro. J Exp Med. 1966 Nov 1;124(5):851–858. doi: 10.1084/jem.124.5.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pinto da Silva P. Translational mobility of the membrane intercalated particles of human erythrocyte ghosts. pH-dependent, reversible aggregation. J Cell Biol. 1972 Jun;53(3):777–787. doi: 10.1083/jcb.53.3.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ray T. K., Lieberman I., Lansing A. I. Synthesis of the plasma membrane of the liver cell. Biochem Biophys Res Commun. 1968 Apr 5;31(1):54–58. doi: 10.1016/0006-291x(68)90030-2. [DOI] [PubMed] [Google Scholar]
  13. Vasiliev J. M., Gelfand I. M., Domnina L. V., Ivanova O. Y., Komm S. G., Olshevskaja L. V. Effect of colcemid on the locomotory behaviour of fibroblasts. J Embryol Exp Morphol. 1970 Nov;24(3):625–640. [PubMed] [Google Scholar]
  14. Vaughan R. B., Trinkaus J. P. Movements of epithelial cell sheets in vitro. J Cell Sci. 1966 Dec;1(4):407–413. doi: 10.1242/jcs.1.4.407. [DOI] [PubMed] [Google Scholar]
  15. Yahara I., Edelman G. M. Restriction of the mobility of lymphocyte immunoglobulin receptors by concanavalin A. Proc Natl Acad Sci U S A. 1972 Mar;69(3):608–612. doi: 10.1073/pnas.69.3.608. [DOI] [PMC free article] [PubMed] [Google Scholar]

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