Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Sep;69(9):2469–2473. doi: 10.1073/pnas.69.9.2469

Modulation of Cell Behavior In Vitro by the Substratum in Fibroblastic and Leukemic Mouse Cell Lines

Alvaro Macieira-Coelho *, Stratis Avrameas †,‡
PMCID: PMC426967  PMID: 4627029

Abstract

Fibroblastic and leukemic mouse cell lines were cultivated on a bovine serum albumin polymer covered with basic or acidic substances. Contact inhibition of fibroblast movement (cell overlapping) and division varied depending on prior treatment of the albumin polymer. The leukemic cells, which normally grow in suspension, attached and spread out on the polymers and could be carried as a monolayer. Preparation of surfaces capable of modulating different parameters of cell behavior in vitro is thus possible.

Keywords: cell membrane, protein polymers, contact inhibition

Full text

PDF

Images in this article

2470Image
on p.2470
2472Image
on p.2472

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABERCROMBIE M., HEAYSMAN J. E. Observations on the social behaviour of cells in tissue culture. II. Monolayering of fibroblasts. Exp Cell Res. 1954 May;6(2):293–306. doi: 10.1016/0014-4827(54)90176-7. [DOI] [PubMed] [Google Scholar]
  2. Baugh C. L., Lecher R. W., Tytell A. A. The effect of pH on the propagation of the diploid cell WI-38 in galactose medium. J Cell Physiol. 1967 Oct;70(2):225–228. doi: 10.1002/jcp.1040700213. [DOI] [PubMed] [Google Scholar]
  3. Carter S. B. Principles of cell motility: the direction of cell movement and cancer invasion. Nature. 1965 Dec 18;208(5016):1183–1187. doi: 10.1038/2081183a0. [DOI] [PubMed] [Google Scholar]
  4. Castor L. N. Flattening, movement and control of division of epithelial-like cells. J Cell Physiol. 1970 Feb;75(1):57–64. doi: 10.1002/jcp.1040750107. [DOI] [PubMed] [Google Scholar]
  5. Ceccarini C., Eagle H. pH as a determinant of cellular growth and contact inhibition. Proc Natl Acad Sci U S A. 1971 Jan;68(1):229–233. doi: 10.1073/pnas.68.1.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dulbecco R. Topoinhibition and serum requirement of transformed and untransformed cells. Nature. 1970 Aug 22;227(5260):802–806. doi: 10.1038/227802a0. [DOI] [PubMed] [Google Scholar]
  7. Eagle H., Foley G. E., Koprowski H., Lazarus H., Levine E. M., Adams R. A. Growth characteristics of virus-transformed cells. Maximum population density, inhibition by normal cells, serum requirement, growth in soft agar, and xenogeneic transplantability. J Exp Med. 1970 Apr 1;131(4):863–879. doi: 10.1084/jem.131.4.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kruse P. F., Jr, Miedema E. Production and characterization of multiple-layered populations of animal cells. J Cell Biol. 1965 Nov;27(2):273–279. doi: 10.1083/jcb.27.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Macieira-Coelho A., Avrameas S. Variations in cell overlapping and growth depending on the substratum. Proc Soc Exp Biol Med. 1972 Apr;139(4):1374–1378. doi: 10.3181/00379727-139-36366. [DOI] [PubMed] [Google Scholar]
  10. Macieira-Coelho A. Dissociation between inhibition of movement and inhibition of division in RSV transformed human fibroblasts. Exp Cell Res. 1967 Aug;47(1):193–200. doi: 10.1016/0014-4827(67)90222-4. [DOI] [PubMed] [Google Scholar]
  11. Macieira-Coelho A. Relationship between DNA synthesis and cell density in normal and virus-transformed cells. Int J Cancer. 1967 Jul 15;2(4):297–303. doi: 10.1002/ijc.2910020404. [DOI] [PubMed] [Google Scholar]
  12. Rubin H. pH and population density in the regulation of animal cell multiplication. J Cell Biol. 1971 Dec;51(3):686–702. doi: 10.1083/jcb.51.3.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ryser H. J., Hancock R. Histones and basic polyamino acids stimulate the uptake of albumin by tumor cells in culture. Science. 1965 Oct 22;150(3695):501–503. doi: 10.1126/science.150.3695.501. [DOI] [PubMed] [Google Scholar]
  14. Todaro G. J., Lazar G. K., Green H. The initiation of cell division in a contact-inhibited mammalian cell line. J Cell Physiol. 1965 Dec;66(3):325–333. doi: 10.1002/jcp.1030660310. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES