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. 1972 Sep;69(9):2495–2499. doi: 10.1073/pnas.69.9.2495

An Active Derivative of Rabbit Antibody Light Chain Composed of the Constant and the Variable Domains Held Together Only by a Native Disulfide Bond

Knud Poulsen 1, Kevin J Fraser 1, Edgar Haber 1
PMCID: PMC426973  PMID: 4506770

Abstract

The immunoglobulin light chain is thought to be organized into two structural domains, the constant and the variable regions, linked by a switch region of lesser tertiary organization. Light chains of homogeneous rabbit antibodies to pneumococcal polysaccharides labeled with 125I were subjected to trypsin digestion at different temperatures. At 43° molecular size (as determined by gel filtration) remained unchanged until the chain was subjected to full reduction and alkylation, when half molecules were demonstrated. One light chain resisted tryptic digestion at 43°. At 50° extensive digestion to small peptides occurred. These experiments confirm the susceptibility of the switch region to enzymatic attack as well as the relative resistance of the constant and variable region domains. They also demonstrate that the third disulfide bridge in rabbit light chain extends between the two structural domains and maintains the integrity of the molecule even when a peptide bond in the switch region is cleaved. When the light chain was subjected to dilute acid hydrolysis [10% acetic acid-pyridine (pH 2.5) in 7 M guanidine-HCl for 24-90 hr] selective cleavage at the switch region was demonstrated in yields up to 90%. The site of cleavage was shown to be the acid labile Asp 109-Pro 110 bond by automated Edman degradation. After cleavage the molecule remained intact until full reduction and alkylation, when half molecules were again demonstrated. A light chain cleaved in this way with its interdomain disulfide bond intact, was able to recombine with a homologous heavy chain and restore antigen binding. This indicates that an intact switch region is not needed for antibody activity. The availability of a functional variable region now allows for planning an examination of the antibody combining site by the technique of peptide synthesis.

Keywords: amino-acid sequence, antibody activity, limited peptide cleavage, antibody combining site

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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