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. 1972 Sep;69(9):2556–2560. doi: 10.1073/pnas.69.9.2556

Antigen-Binding Specificity of Isolated Cell-Surface Immunoglobulin from Thymus Cells Activated to Histocompatibility Antigens

Robert E Cone 1, Jon Sprent 1, John J Marchalonis 1
PMCID: PMC426987  PMID: 4506774

Abstract

Lactoperoxidase-catalyzed radioiodination of cell-surface proteins was used in the isolation of cell-surface immunoglobulin from thymus-derived thoracic duct lymphocytes activated to histocompatibility-2 antigens. Immunoglobulin was identified by specific precipitation with antiserum to mouse immunoglobulin. Polyacrylamide gel electrophoresis of reduced and alkylated precipitates showed that the immunoglobulin molecules possessed μ-type heavy chains and light chains. Cell-surface immunoglobulin isolated from thymus-derived cells activated to histocompatibility-2 antigens possessed binding specificity for the activating antigens.

Keywords: mouse T cells, 125I, precipitation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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