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. 1972 Sep;69(9):2627–2631. doi: 10.1073/pnas.69.9.2627

Regeneration of Cation-Transport Capacity in HeLa Cell Membranes After Specific Blockade by Ouabain

Gerald L Vaughan 1,2,*, John S Cook 1,2
PMCID: PMC427003  PMID: 4506784

Abstract

The cardiac glycoside, ouabain, inhibits alkali-cation transport in HeLa cells. It binds to 0.75 × 106 sites per cell, and the half-time for its dissociation is 16 hr. After partial blockade by ouabain, the cell generates new ouabain-binding sites, with total restoration of transport in 10% of a cell cycle(∼3 hr). This recovery requires protein synthesis and appears to be a response to altered cell-electrolyte content, since growth of cells in media with low K+ concentration enhances the titer of the transport enzyme in a fashion similar to the effect of ouabain. Totally blocked cells do not recover.

Keywords: ion transport, repair, cardiac glycoside, plasma membrane

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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