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. 1972 Sep;69(9):2727–2731. doi: 10.1073/pnas.69.9.2727

In Vitro Synthesis of Procollagen on Polysomes

S S Kerwar *, Leonard D Kohn †, Charles M Lapiere ‡, Herbert Weissbach *
PMCID: PMC427026  PMID: 4341706

Abstract

The major collagenous products synthesized in a cell-free polysome preparation are pro-α1 and pro-α2, formed in a ratio of 2:1. They are the precursor forms of α1 and α2 chains of normal connective-tissue collagen that are also formed in small amounts. A procollagen peptidase activity has been demonstrated in the supernatant fraction that can account for the formation of α1 and α2 chains from their precursors. The polysomal system is activated by a salt extract of reticulocyte ribosomes and is inhibited by aurintricarboxylic acid, suggesting that the polysomes are able to initiate protein synthesis.

Keywords: collagen, protein synthesis, mRNA, initiation factors

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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