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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Sep;69(9):2745–2749. doi: 10.1073/pnas.69.9.2745

Distribution of Pyrimidine Oligonucleotides in Strands L and H of Bacillus subtilis DNA

Rivka Rudner 1, Mary Ledoux 1, Anne Mazelis 1
PMCID: PMC427031  PMID: 4341707

Abstract

The distribution of pyrimidine oligodeoxynucleotide clusters in L and H strands of Bacillus subtilis DNA separated by methylated albumin-Kieselguhr has been determined. Preparations of native and single-stranded DNA were degraded with diphenylamine in formic acid, and the released isostichs with the general formula of PynPn+1 were separated on DEAE-cellulose by chain length. Eleven isostichs were obtained for strands L and H in unequal proportions. Each isostich fraction was subfractionated by base composition on DEAE-cellulose at pH 3.0. 61 Pyrimidine oligonucleotide clusters were separated from the H strand and only 46 from the L strand. The findings show a higher degree of asymmetry between the strands in the distribution of cytosine-rich clusters as compared with thymine-rich clusters. The longest cytosine oligodeoxynucleotide present in both strands is of chain length 5. There is an unusually high distribution of thymine oligodeoxynucleotides of length 5-11. Up to chain length 6, the distribution of thymine oligodeoxynucleotides between the strands is about equal; from chain length 7 to 11 they occur predominantly in the H strand.

Keywords: methylated albumin-Kieselguhr chromatography; isostich analysis; oligo(dC,dT) clusters

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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