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. 1973 Sep;70(9):2544–2548. doi: 10.1073/pnas.70.9.2544

Separation of the Messenger RNAs of Newcastle Disease Virus by Gel Electrophoresis

Bonnie Spanier Collins 1, Michael A Bratt 1
PMCID: PMC427052  PMID: 4517668

Abstract

We have separated the 18-22S putative messenger RNA of Newcastle disease virus into seven species ranging in molecular weight from 0.55 to 1.53 × 106 using sodium dodecyl sulfate-acrylamide-gel electrophoresis at relatively high concentrations of acrylamide and for a relatively long time. Studies of the number and molecular weights of the proteins and the 18-22S RNAs of the virus suggests that these RNAs are in the right molecular weight range to code for the known proteins of Newcastle disease virus. In preliminary studies using this separation technique, we have demonstrated that: (a) there is no difference between the 18-22S RNA made during a normal infection and when genome replication is blocked; and (b) there is a strain-specific difference between the RNAs of Newcastle disease virus-AV and Newcastle disease virus-HP.

Keywords: early and late RNA, strain comparisons

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attardi G., Amaldi F. Structure and synthesis of ribosomal RNA. Annu Rev Biochem. 1970;39:183–226. doi: 10.1146/annurev.bi.39.070170.001151. [DOI] [PubMed] [Google Scholar]
  2. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  4. Bratt M. A., Gallaher W. R. Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proc Natl Acad Sci U S A. 1969 Oct;64(2):536–543. doi: 10.1073/pnas.64.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bratt M. A. RNA synthesis in chick embryo cells infected with different strains of NDV. Virology. 1969 Jul;38(3):485–488. doi: 10.1016/0042-6822(69)90163-9. [DOI] [PubMed] [Google Scholar]
  6. Bratt M. A., Robinson W. S. Evidence for an RNA replicative intermediate in cells infected with Newcastle disease virus. J Gen Virol. 1971 Feb;10(2):139–145. doi: 10.1099/0022-1317-10-2-139. [DOI] [PubMed] [Google Scholar]
  7. Clavell L. A., Bratt M. A. Relationship between the ribonucleic acid synthesizing capacity of ultraviolet-irradiated Newcastle disease virus and its ability to induce interferon. J Virol. 1971 Oct;8(4):500–508. doi: 10.1128/jvi.8.4.500-508.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Darnell J. E., Jr Ribonucleic acids from animal cells. Bacteriol Rev. 1968 Sep;32(3):262–290. doi: 10.1128/br.32.3.262-290.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Duesberg P. H., Robinson W. S. Isolation of the nucleic acid of Newcastle disease virus (NDV). Proc Natl Acad Sci U S A. 1965 Sep;54(3):794–800. doi: 10.1073/pnas.54.3.794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Duesberg P. H. The RNA of influenza virus. Proc Natl Acad Sci U S A. 1968 Mar;59(3):930–937. doi: 10.1073/pnas.59.3.930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kingsbury D. W. Newcastle disease virus RNA. I. Isolation and preliminary characterization of RNA from virus particles. J Mol Biol. 1966 Jun;18(1):195–203. doi: 10.1016/s0022-2836(66)80085-2. [DOI] [PubMed] [Google Scholar]
  13. Kingsbury D. W. Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. J Mol Biol. 1966 Jun;18(1):204–214. doi: 10.1016/s0022-2836(66)80086-4. [DOI] [PubMed] [Google Scholar]
  14. Kingsbury D. W., Portner A., Darlington R. W. Properties of incomplete Sendai virions and subgenomic viral RNAs. Virology. 1970 Dec;42(4):857–871. doi: 10.1016/0042-6822(70)90335-1. [DOI] [PubMed] [Google Scholar]
  15. Lomniczi B., Meager A., Burke D. C. Virus RNA and protein synthesis in cells infected with different strains of Newcastle disease virus. J Gen Virol. 1971 Oct;13(1):111–120. doi: 10.1099/0022-1317-13-1-111. [DOI] [PubMed] [Google Scholar]
  16. Palmiter R. D., Palacios R., Schimke R. T. Identification and isolation of ovalbumin-synthesizing polysomes. II. Quantification and immunoprecipitation of polysomes. J Biol Chem. 1972 May 25;247(10):3296–3304. [PubMed] [Google Scholar]
  17. Pene J. J., Knight E., Jr, Darnell J. E., Jr Characterization of a new low molecular weight RNA in HeLa cell ribosomes. J Mol Biol. 1968 May 14;33(3):609–623. doi: 10.1016/0022-2836(68)90309-4. [DOI] [PubMed] [Google Scholar]
  18. Robinson W. S. Sendai virus RNA synthesis and nucleocapsid formation in the presence of cycloheximide. Virology. 1971 Jun;44(3):494–502. doi: 10.1016/0042-6822(71)90362-x. [DOI] [PubMed] [Google Scholar]
  19. Shatkin A. J., Rada B. Reovirus-directed ribonucleic acid synthesis in infected L cells. J Virol. 1967 Feb;1(1):24–35. doi: 10.1128/jvi.1.1.24-35.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Watanabe Y., Prevec L., Graham A. F. Specificity in transcription of the reovirus genome. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1040–1046. doi: 10.1073/pnas.58.3.1040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zhdanov V. M. Primenenie poliakrilamidnyh gelei dlia izucheniia RNK virusa bolezni n'iukasla. Vopr Virusol. 1969 Jan-Feb;14(1):37–41. [PubMed] [Google Scholar]

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