Abstract
The template activity of RNA extracted from unfertilized sea urchin eggs was demonstrated in a cell-free translation system, and, for the first time, specific proteins were identified among the products. All messenger RNA activity is recovered, under the conditions used, from the postribosomal supernatant. Histones, among many other proteins, were identified specifically as products of this “maternal” messenger RNA.
Keywords: histones, cell-free synthesis
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Selected References
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- Baltimore D., Huang A. S. Interaction of HeLa cell proteins with RNA. J Mol Biol. 1970 Feb 14;47(3):263–273. doi: 10.1016/0022-2836(70)90301-3. [DOI] [PubMed] [Google Scholar]
- Blobel G. Protein tightly bound to globin mRNA. Biochem Biophys Res Commun. 1972 Apr 14;47(1):88–95. doi: 10.1016/s0006-291x(72)80014-7. [DOI] [PubMed] [Google Scholar]
- Borun T. W., Scharff M. D., Robbins E. Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1977–1983. doi: 10.1073/pnas.58.5.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crane C. M., Villee C. A. The synthesis of nuclear histones in early embryogenesis. J Biol Chem. 1971 Feb 10;246(3):719–723. [PubMed] [Google Scholar]
- Davidson E. H., Crippa M., Kramer F. R., Mirsky A. E. Genomic function during the lampbrush chromosome stage of amphibian oogenesis. Proc Natl Acad Sci U S A. 1966 Sep;56(3):856–863. doi: 10.1073/pnas.56.3.856. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GROSS P. R., COUSINEAU G. H. MACROMOLECULE SYNTHESIS AND THE INFLUENCE OF ACTINOMYCIN ON EARLY DEVELOPMENT. Exp Cell Res. 1964 Feb;33:368–395. doi: 10.1016/0014-4827(64)90002-3. [DOI] [PubMed] [Google Scholar]
- Gross K., Ruderman J., Jacobs-Lorena M., Baglioni C., Gross P. R. Cell-free synthesis of histones directed by messenger RNA from sea urchin embryos. Nat New Biol. 1973 Feb 28;241(113):272–274. doi: 10.1038/newbio241272a0. [DOI] [PubMed] [Google Scholar]
- Gross P. R., Malkin L. I., Hubbard M. Synthesis of RNA during oogenesis in the sea urchin. J Mol Biol. 1965 Sep;13(2):463–481. doi: 10.1016/s0022-2836(65)80110-3. [DOI] [PubMed] [Google Scholar]
- Henshaw E. C., Loebenstein J. Rapidly labeled, polydisperse RNA in rat-liver cytoplasm: evidence that it is contained in ribonucleoprotein particles of heterogeneous size. Biochim Biophys Acta. 1970 Feb 18;199(2):405–420. doi: 10.1016/0005-2787(70)90083-3. [DOI] [PubMed] [Google Scholar]
- Hogan B., Gross P. R. The effect of protein synthesis inhibition on the entry of messenger RNA into the cytoplasm of sea urchin embryos. J Cell Biol. 1971 Jun;49(3):692–701. doi: 10.1083/jcb.49.3.692. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Infante A. A., Nemer M. Heterogeneous ribonucleoprotein particles in the cytoplasm of sea urchin embryos. J Mol Biol. 1968 Mar 28;32(3):543–565. doi: 10.1016/0022-2836(68)90342-2. [DOI] [PubMed] [Google Scholar]
- Jacobs-Lorena M., Baglioni C., Borun T. W. Translation of messenger RNA for histones from HeLa cells by a cell-free extract from mouse ascites tumor. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2095–2099. doi: 10.1073/pnas.69.8.2095. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jacobs-Lorena M., Baglioni C. Characterization of a mouse ascites cell-free system. Biochemistry. 1972 Dec 19;11(26):4970–4974. doi: 10.1021/bi00776a015. [DOI] [PubMed] [Google Scholar]
- Jacobs-Lorena M., Baglioni C. Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1425–1428. doi: 10.1073/pnas.69.6.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kafatos F. C. Cytoplasmic particles carrying rapidly labeled RNA in developing insect epidermis. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1251–1258. doi: 10.1073/pnas.59.4.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kedes L. H., Gross P. R. Identification in cleaving embryos of three RNA species serving as templates for the synthesis of nuclear proteins. Nature. 1969 Sep 27;223(5213):1335–1339. doi: 10.1038/2231335a0. [DOI] [PubMed] [Google Scholar]
- Kedes L. H., Gross P. R. Synthesis and function of messenger RNA during early embryonic development. J Mol Biol. 1969 Jun 28;42(3):559–575. doi: 10.1016/0022-2836(69)90243-5. [DOI] [PubMed] [Google Scholar]
- Kedes L. H., Hogan B., Cognetti G., Selvig S., Yanover P., Gross P. R. Regulation of translation and transcription of messenger RNA during early embryonic development. Cold Spring Harb Symp Quant Biol. 1969;34:717–723. doi: 10.1101/sqb.1969.034.01.081. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Maggio R., Vittorelli M. L., Rinaldi A. M., Monroy A. In vitro incorporation of amino acids into proteins stimulated by RNA from unfertilized sea urchin eggs. Biochem Biophys Res Commun. 1964 Apr 22;15(5):436–441. doi: 10.1016/0006-291x(64)90481-4. [DOI] [PubMed] [Google Scholar]
- Mano Y., Nagano H. Release of maternal RNA from some particles as a mechanism of activation of protein synthesis fertilization in sea urchin eggs. Biochem Biophys Res Commun. 1966 Oct 20;25(2):210–215. doi: 10.1016/0006-291x(66)90582-1. [DOI] [PubMed] [Google Scholar]
- Mathews M., Korner A. Mammalian cell-free protein synthesis directed by viral ribonucleic acid. Eur J Biochem. 1970 Dec;17(2):328–338. doi: 10.1111/j.1432-1033.1970.tb01170.x. [DOI] [PubMed] [Google Scholar]
- Panyim S., Chalkley R. High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969 Mar;130(1):337–346. doi: 10.1016/0003-9861(69)90042-3. [DOI] [PubMed] [Google Scholar]
- Perry R. P., Kelley D. E. Messenger RNA-protein complexes and newly synthesized ribosomal subunits: analysis of free particles and components of polyribosomes. J Mol Biol. 1968 Jul 14;35(1):37–59. doi: 10.1016/s0022-2836(68)80035-x. [DOI] [PubMed] [Google Scholar]
- Piatigorsky J., Tyler A. Changes upon fertilization in the distribution of RNA-containing particles in sea urchin eggs. Dev Biol. 1970 Feb;21(1):13–28. doi: 10.1016/0012-1606(70)90058-8. [DOI] [PubMed] [Google Scholar]
- Raff R. A., Colot H. V., Selvig S. E., Gross P. R. Oogenetic origin of messenger RNA for embryonic synthesis of microtubule proteins. Nature. 1972 Jan 28;235(5335):211–214. doi: 10.1038/235211a0. [DOI] [PubMed] [Google Scholar]
- Schultz G. A., Chen D., Katchalski E. Localization of a messenger RNA in a ribosomal fraction from ungerminated wheat embryos. J Mol Biol. 1972 May 28;66(3):379–390. doi: 10.1016/0022-2836(72)90421-4. [DOI] [PubMed] [Google Scholar]
- Slater D. W., Spiegelman S. An estimation of genetic messages in the unfertilized echinoid egg. Proc Natl Acad Sci U S A. 1966 Jul;56(1):164–170. doi: 10.1073/pnas.56.1.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slater I., Gillespie D., Slater D. W. Cytoplasmic adenylylation and processing of maternal RNA. Proc Natl Acad Sci U S A. 1973 Feb;70(2):406–411. doi: 10.1073/pnas.70.2.406. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith A. E., Marcker K. A., Mathews M. B. Translation of RNA from encephalomyocarditis virus in a mammalian cell-free system. Nature. 1970 Jan 10;225(5228):184–187. doi: 10.1038/225184a0. [DOI] [PubMed] [Google Scholar]
- Spirin A. S., Nemer M. Messenger RNA in early sea-urchin embryos: cytoplasmic particles. Science. 1965 Oct 8;150(3693):214–217. doi: 10.1126/science.150.3693.214. [DOI] [PubMed] [Google Scholar]
- Spirin A. S. The second Sir Hans Krebs Lecture. Informosomes. Eur J Biochem. 1969 Aug;10(1):20–35. doi: 10.1111/j.1432-1033.1969.tb00651.x. [DOI] [PubMed] [Google Scholar]
- Spohr G., Granboulan N., Morel C., Scherrer K. Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy. Eur J Biochem. 1970 Dec;17(2):296–318. doi: 10.1111/j.1432-1033.1970.tb01168.x. [DOI] [PubMed] [Google Scholar]
- Stavy L., Gross P. R. Availability of mRNA for translation during normal and transcription-blocked development. Biochim Biophys Acta. 1969 May 20;182(1):203–213. doi: 10.1016/0005-2787(69)90535-8. [DOI] [PubMed] [Google Scholar]