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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Sep;70(9):2633–2636. doi: 10.1073/pnas.70.9.2633

Enzyme Assay in Microsomes Below Zero Degrees

Pascale Debey 1, Claude Balny 1, Pierre Douzou 1
PMCID: PMC427071  PMID: 4147450

Abstract

Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0° were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and some individual and sequential enzyme assays can be performed over a wide range of temperatures below 0°.

Keywords: ethylene glycol, electron transfer, rats, liver

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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