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. 1973 Sep;70(9):2651–2655. doi: 10.1073/pnas.70.9.2651

Regulation of Subunit Synthesis of Escherichia coli RNA Polymerase

Joel B Kirschbaum 1,*
PMCID: PMC427075  PMID: 4126266

Abstract

λcI857s7drifd18 phage is a defective, specialized transducing phage carrying the RNA polymerase mutation rifd, a dominant rifampicin-resistant allele. This phage DNA directs in vitro synthesis of the β subunit of RNA polymerase in a DNA-dependent protein-synthesizing system. Prophage induction in cells that are lysogenic for this phage increases the rate of β-subunit synthesis in vivo by at most 2-fold. No increase in subunit synthesis above the haploid level is observed in cells diploid for the β gene.

Keywords: phage λ, antibody, in vitro synthesis

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Selected References

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