Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Sep;70(9):2651–2655. doi: 10.1073/pnas.70.9.2651

Regulation of Subunit Synthesis of Escherichia coli RNA Polymerase

Joel B Kirschbaum 1,*
PMCID: PMC427075  PMID: 4126266

Abstract

λcI857s7drifd18 phage is a defective, specialized transducing phage carrying the RNA polymerase mutation rifd, a dominant rifampicin-resistant allele. This phage DNA directs in vitro synthesis of the β subunit of RNA polymerase in a DNA-dependent protein-synthesizing system. Prophage induction in cells that are lysogenic for this phage increases the rate of β-subunit synthesis in vivo by at most 2-fold. No increase in subunit synthesis above the haploid level is observed in cells diploid for the β gene.

Keywords: phage λ, antibody, in vitro synthesis

Full text

PDF
2651

Images in this article

2652Image
on p.2652

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austin S., McGeoch D. The synthesis in vitro of RNA polymerase subunits of Escherichia coli. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2420–2423. doi: 10.1073/pnas.70.8.2420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Austin S., Scaife J. A new method for selecting RNA polymerase mutants. J Mol Biol. 1970 Apr 14;49(1):263–267. doi: 10.1016/0022-2836(70)90394-3. [DOI] [PubMed] [Google Scholar]
  3. Beckwith J. R., Signer E. R., Epstein W. Transposition of the Lac region of E. coli. Cold Spring Harb Symp Quant Biol. 1966;31:393–401. doi: 10.1101/sqb.1966.031.01.051. [DOI] [PubMed] [Google Scholar]
  4. Berg D., Chamberlin M. Physical studies on ribonucleic acid polymerase from Escherichia coli B. Biochemistry. 1970 Dec 22;9(26):5055–5064. doi: 10.1021/bi00828a003. [DOI] [PubMed] [Google Scholar]
  5. Burgess R. R. Separation and characterization of the subunits of ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6168–6176. [PubMed] [Google Scholar]
  6. Buxton R. S. Genetic analysis of Escherichia coli K12 mutants resistant to bacteriophage BF23 and the E-group colicins. Mol Gen Genet. 1971;113(2):154–156. doi: 10.1007/BF00333188. [DOI] [PubMed] [Google Scholar]
  7. Hayward R. S., Tittawella I. P., Scaife J. G. Evidence for specific control of RNA polymerase synthesis in Escherichia coli. Nat New Biol. 1973 May 2;243(122):6–9. [PubMed] [Google Scholar]
  8. Heil A., Zillig W. Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription. FEBS Lett. 1970 Dec;11(3):165–168. doi: 10.1016/0014-5793(70)80519-1. [DOI] [PubMed] [Google Scholar]
  9. Matzura H., Molin S., Maaloe O. Sequential biosynthesis of the and ' subunits of the DNA-dependent RNA polymerase from Escherichia coli. J Mol Biol. 1971 Jul 14;59(1):17–25. doi: 10.1016/0022-2836(71)90410-4. [DOI] [PubMed] [Google Scholar]
  10. Müller-Hill B., Crapo L., Gilbert W. Mutants that make more lac repressor. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1259–1264. doi: 10.1073/pnas.59.4.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Platt T., Miller J. H., Weber K. In vivo degradation of mutant lac repressor. Nature. 1970 Dec 19;228(5277):1154–1156. doi: 10.1038/2281154a0. [DOI] [PubMed] [Google Scholar]
  12. Shapiro J., Machattie L., Eron L., Ihler G., Ippen K., Beckwith J. Isolation of pure lac operon DNA. Nature. 1969 Nov 22;224(5221):768–774. doi: 10.1038/224768a0. [DOI] [PubMed] [Google Scholar]
  13. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES