Abstract
We have previously shown that neoplastic cells of human breast cancers, leukemias, lymphomas, and sarcomas contain particles similar to the viruses that have been established as etiologic agents of these diseases in mice. The present paper concerns tumors of the central nervous system for which no suitable animal model or corresponding virus exists. Nevertheless, using the simultaneous detection test, we showed that human brain tumors contain 70S RNA and RNA-directed DNA polymerase encapsulated in a particulate component possessing a density of 1.17 g/ml. These particles satisfy the three diagnostic criteria that characterize RNA tumor viruses of animals. 24 Out of 26 (92%) of the most malignant (glioblastoma and medulloblastoma) brain tumors examined contained these virus-like entities.
Keywords: simultaneous detection test, oncornaviruses
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Axel R., Gulati S. C., Spiegelman S. Particles containing RNA-instructed DNA polymerase and virus-related RNA in human breast cancers. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3133–3137. doi: 10.1073/pnas.69.11.3133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Axel R., Schlom J., Spiegelman S. Presence in human breast cancer of RNA homologous to mouse mammary tumour virus RNA. Nature. 1972 Jan 7;235(5332):32–36. doi: 10.1038/235032a0. [DOI] [PubMed] [Google Scholar]
- Baxt W., Hehlmann R., Spiegelman S. Human leukaemic cells contain reverse transcriptase associated with a high molecular weight virus-related RNA. Nat New Biol. 1972 Nov 15;240(98):72–75. doi: 10.1038/newbio240072a0. [DOI] [PubMed] [Google Scholar]
- Gulati S. C., Axel R., Spiegelman S. Detection of RNA-instructed DNA polymerase and high molecular weight RNA in malignant tissue. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2020–2024. doi: 10.1073/pnas.69.8.2020. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALL B. D., SPIEGELMAN S. Sequence complementarity of T2-DNA and T2-specific RNA. Proc Natl Acad Sci U S A. 1961 Feb 15;47:137–163. doi: 10.1073/pnas.47.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hehlmann R., Kufe D., Spiegelman S. RNA in human leukemic cells related to the RNA of a mouse leukemia virus (leukocytes-RNA-DNA hybridization-rauscher virus-polysomal RNA). Proc Natl Acad Sci U S A. 1972 Feb;69(2):435–439. doi: 10.1073/pnas.69.2.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hehlmann R., Kufe D., Spiegelman S. Viral-related RNA in Hodgkins' disease and other human lymphomas. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1727–1731. doi: 10.1073/pnas.69.7.1727. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kufe D., Hehlmann R., Spiegelman S. Human sarcomas contain RNA related to the RNA of a mouse leukemia virus. Science. 1972 Jan 14;175(4018):182–185. doi: 10.1126/science.175.4018.182. [DOI] [PubMed] [Google Scholar]
- ROGERS J. B., BLUMENTHAL H. T. Studies of guinea pig tumors. I. Report of fourteen spontaneous guinea pig tumors, with a review of the literature. Cancer Res. 1960 Feb;20:191–197. [PubMed] [Google Scholar]
- Schlom J., Spiegelman S. Simultaneous detection of reverse transcriptase and high molecular weight RNA unique to oncogenic RNA viruses. Science. 1971 Nov 19;174(4011):840–843. doi: 10.1126/science.174.4011.840. [DOI] [PubMed] [Google Scholar]
- Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Travnicek M., Watson K. Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses. Nature. 1970 Aug 8;227(5258):563–567. doi: 10.1038/227563a0. [DOI] [PubMed] [Google Scholar]