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. 1973 Oct;70(10):2823–2826. doi: 10.1073/pnas.70.10.2823

Methionine Transfer Ribonucleic Acids of Avian Myeloblastosis Virus

Kay T Elder *, Alan E Smith †
PMCID: PMC427117  PMID: 4355368

Abstract

Chick-embryo cells contain four isoaccepting species of methionine transfer RNA (I-IV). One species (I) is the initiator, tRNAfMet, and the others (II, III, and IV) are the donors of internal methionyl residues (tRNAmMet). Over 85% of the tRNAMet in purified avian myeloblastosis virus consists of one tRNAmMet species, which resembles host-cell tRNAMet IV with respect to chromatographic properties on RPC-5, electrophoretic mobility of the terminal methionyl-oligonucleotide, and activity in cell-free protein synthesis in response to synthetic and natural messenger RNAs. Thus, tRNAMet IV of avian myeloblastosis virus is structurally indistinguishable from the corresponding host-cell species, and, although not involved in initiation of viral protein synthesis, is able to function in peptide-chain elongation.

Keywords: RPC-5 chromatography, in vitro protein synthesis, initiator tRNA, encephalomyocarditis virus RNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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