Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Oct;70(10):2946–2950. doi: 10.1073/pnas.70.10.2946

Survey of DNA Polymerase Activity During the Early Development of Drosophila melanogaster

Lola Margulies 1, Erwin Chargaff 1
PMCID: PMC427144  PMID: 4200726

Abstract

The pattern of DNA polymerase activity in developing Drosophila melanogaster has been studied in seven stages of embryonic development as well as in unfertilized eggs. The crude polymerase-containing extracts, most likely of cytoplasmic origin, utilize, in the following order of decreasing template efficiency, “activated” calfthymus DNA, poly(A)·oligo(dT), and poly(A)·oligo(U). The highest enzymes levels occur in unfertilized eggs; the activity remains high during the first 9 hr of embryogenesis, but shows a progressive decline in the later stages. Deoxyribonuclease exhibits a similar trend. The unfertilized eggs of two genotypically different females had nearly identical levels of DNA polymerase.

Full text

PDF
2946

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang L. M., Bollum F. J. Deoxynucleotide-polymerizing enzymes of calf thymus gland. V. Homogeneous terminal deoxynucleotidyl transferase. J Biol Chem. 1971 Feb 25;246(4):909–916. [PubMed] [Google Scholar]
  2. Kasai K., Grunberg-Manago M. Sheep kidney nuclease. Hydrolysis of tRNA. Eur J Biochem. 1967 Apr;1(2):152–163. doi: 10.1007/978-3-662-25813-2_24. [DOI] [PubMed] [Google Scholar]
  3. Knippers R. DNA polymerase II. Nature. 1970 Dec 12;228(5276):1050–1053. doi: 10.1038/2281050a0. [DOI] [PubMed] [Google Scholar]
  4. MASTER R. W. POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES. Nature. 1965 Apr 3;206:93–93. doi: 10.1038/206093b0. [DOI] [PubMed] [Google Scholar]
  5. Muhammed A., Gonçalves J. M., Trosko J. E. Deoxyribonuclease and deoxyribonucleic acid polymerase activity during Drosophila development. Dev Biol. 1967 Jan;15(1):23–32. doi: 10.1016/0012-1606(67)90003-6. [DOI] [PubMed] [Google Scholar]
  6. NIGON V., DAILLIE J. La synthèse de l'acide désoxyribonucléique au cours du développement de la drosophile. Biochim Biophys Acta. 1958 Aug;29(2):246–255. doi: 10.1016/0006-3002(58)90181-1. [DOI] [PubMed] [Google Scholar]
  7. Rasch E. M., Barr H. J., Rasch R. W. The DNA content of sperm of Drosophila melanogaster. Chromosoma. 1971;33(1):1–18. doi: 10.1007/BF00326379. [DOI] [PubMed] [Google Scholar]
  8. SCHULTZ J. The relation of the heterochromatic chromosome regions to the nucleic acids of the cell. Cold Spring Harb Symp Quant Biol. 1956;21:307–328. doi: 10.1101/sqb.1956.021.01.025. [DOI] [PubMed] [Google Scholar]
  9. Stavrianopoulos J. G., Karkas J. D., Chargaff E. DNA polymerase of chicken embryo: purification and properties. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1781–1785. doi: 10.1073/pnas.69.7.1781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stavrianopoulos J. G., Karkas J. D., Chargaff E. Nucleic acid polymerases of the developing chicken embryo: a DNA polymerase preferring a hybrid template. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2207–2211. doi: 10.1073/pnas.68.9.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES