Abstract
Friend leukemia cells grown in the presence of dimethyl sulfoxide show enhanced erythroid differentiation and hemoglobin synthesis. The effects of dimethyl sulfoxide stimulation are inhibited by BrdU. To determine the effect of BrdU on the amount of globin mRNA present in cells treated and not treated with dimethyl sulfoxide, molecular hybridization between total cell RNA and [3H]DNA complementary to mouse globin mRNA was used. Cells treated with BrdU and dimethyl sulfoxide had 70% less globin mRNA than cells treated with dimethyl sulfoxide alone. The size and base sequence of the residual globin mRNA in the cultures treated with BrdU and dimethyl sulfoxide were unaltered. Cells treated with BrdU alone contained slightly more globin mRNA than did the untreated controls, suggesting that BrdU may have a dual effect in transcription of messenger RNA.
Keywords: differentiation, Friend leukemia virus
Full text
PDF![2956](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f892/427146/599679dc02a1/pnas00137-0246.png)
![2957](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f892/427146/f6168422cae3/pnas00137-0247.png)
![2958](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f892/427146/13309ac50b8e/pnas00137-0248.png)
![2959](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f892/427146/c982b94fbac4/pnas00137-0249.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aaronson S. A., Todaro G. J., Scolnick E. M. Induction of murine C-type viruses from clonal lines of virus-free BALB-3T3 cells. Science. 1971 Oct 8;174(4005):157–159. doi: 10.1126/science.174.4005.157. [DOI] [PubMed] [Google Scholar]
- Ando T. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae. Biochim Biophys Acta. 1966 Jan 18;114(1):158–168. doi: 10.1016/0005-2787(66)90263-2. [DOI] [PubMed] [Google Scholar]
- Baltimore D., Smoler D. F. Association of an endoribonuclease with the avian myeloblastosis virus deoxyribonucleic acid polymerase. J Biol Chem. 1972 Nov 25;247(22):7282–7287. [PubMed] [Google Scholar]
- Benz E. J., Jr, Forget B. G. Defect in messenger RNA for human hemoglobin synthesis in beta thalassemia. J Clin Invest. 1971 Dec;50(12):2755–2760. doi: 10.1172/JCI106778. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bischoff R., Holtzer H. Inhibition of myoblast fusion after one round of DNA synthesis in 5-bromodeoxyuridine. J Cell Biol. 1970 Jan;44(1):134–150. doi: 10.1083/jcb.44.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FRAENKEL-CONRAT H., SINGER B., TSUGITA A. Purification of viral RNA by means of bentonite. Virology. 1961 May;14:54–58. doi: 10.1016/0042-6822(61)90131-3. [DOI] [PubMed] [Google Scholar]
- Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hagopian H. K., Lippke J. A., Ingram V. M. Erythropoietic cell cultures from chick embryos. J Cell Biol. 1972 Jul;54(1):98–106. doi: 10.1083/jcb.54.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Housman D., Forget B. G., Skoultchi A., Benz E. J., Jr Quantitative deficiency of chain-specific globin messenger ribonucleic acids in the thalassemia syndromes. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1809–1813. doi: 10.1073/pnas.70.6.1809. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klement V., Nicolson M. O., Huebner R. J. Rescue of the genome of focus forming virus from rat non-productive lines by 5'-bromodeoxyruidine. Nat New Biol. 1971 Nov 3;234(44):12–14. doi: 10.1038/newbio234012a0. [DOI] [PubMed] [Google Scholar]
- Lin S. Y., Riggs A. D. Lac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2574–2576. doi: 10.1073/pnas.69.9.2574. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lowy D. R., Rowe W. P., Teich N., Hartley J. W. Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science. 1971 Oct 8;174(4005):155–156. doi: 10.1126/science.174.4005.155. [DOI] [PubMed] [Google Scholar]
- Lyons M. J., Lasfargues E. Y., Came P. E. Effect of actinomycin D and halogenated deoxyuridines on the replication of Bittner virus in vitro. Nature. 1966 Oct 1;212(5057):100–101. doi: 10.1038/212100a0. [DOI] [PubMed] [Google Scholar]
- McCarthy B. J., Church R. B. The specificity of molecular hybridization reactions. Annu Rev Biochem. 1970;39:131–150. doi: 10.1146/annurev.bi.39.070170.001023. [DOI] [PubMed] [Google Scholar]
- Natta C., Banks J., Niazi G., Marks P. A., Bank A. Decreased beta globin mRNA activity in bone marrow cells in homozygous and heterozygous beta thalassaemia. Nat New Biol. 1973 Aug 29;244(139):280–281. doi: 10.1038/newbio244280a0. [DOI] [PubMed] [Google Scholar]
- Ostertag W., Crozier T., Kluge N., Melderis H., Dube S. Action of 5-bromodeoxyuridine on the induction of haemoglobin synthesis in mouse leukaemia cells resistant to 5-BUdR. Nat New Biol. 1973 Jun 13;243(128):203–205. doi: 10.1038/newbio243203a0. [DOI] [PubMed] [Google Scholar]
- Pasztor L. M., Hu F. An amelanotic variant of B16 malignant melanoma. Cancer Res. 1972 Aug;32(8):1769–1774. [PubMed] [Google Scholar]
- Ross J., Ikawa Y., Leder P. Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3620–3623. doi: 10.1073/pnas.69.12.3620. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scher W., Preisler H. D., Friend C. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro. 3. Effects of 5-bromo-2'-deoxyuridine, dimethylformamide and dimethylsulfoxide. J Cell Physiol. 1973 Feb;81(1):63–70. doi: 10.1002/jcp.1040810108. [DOI] [PubMed] [Google Scholar]
- Schubert D., Jacob F. 5-bromodeoxyuridine-induced differentiation of a neuroblastoma. Proc Natl Acad Sci U S A. 1970 Sep;67(1):247–254. doi: 10.1073/pnas.67.1.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silagi S., Bruce S. A. Suppression of malignancy and differentiation in melanotic melanoma cells. Proc Natl Acad Sci U S A. 1970 May;66(1):72–78. doi: 10.1073/pnas.66.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stellwagen R. H., Tomkins G. M. Preferential inhibition by 5-bromodeoxyuridine of the synthesis of tyrosine aminotransferase in hepatoma cell cultures. J Mol Biol. 1971 Feb 28;56(1):167–182. doi: 10.1016/0022-2836(71)90092-1. [DOI] [PubMed] [Google Scholar]
- Sutton W. D. A crude nuclease preparation suitable for use in DNA reassociation experiments. Biochim Biophys Acta. 1971 Jul 29;240(4):522–531. doi: 10.1016/0005-2787(71)90709-x. [DOI] [PubMed] [Google Scholar]
- Teich N., Lowy D. R., Hartley J. W., Rowe W. P. Studies of the mechanism of induction of infectious murine leukemia virus from AKR mouse embryo cell lines by 5-iododeoxyuridine and 5-bromodeoxyuridine. Virology. 1973 Jan;51(1):163–173. doi: 10.1016/0042-6822(73)90376-0. [DOI] [PubMed] [Google Scholar]
- Turkington R. W., Majumder G. C., Riddle M. Inhibition of mammary gland differentiation in vitro by 5-bromo-2'-deoxyuridine. J Biol Chem. 1971 Mar 25;246(6):1814–1819. [PubMed] [Google Scholar]
- Verma I. M., Meuth N. L., Bromfeld E., Manly K. F., Baltimore D. Covalently linked RNA-DNA molecule as initial product of RNA tumour virus DNA polymerase. Nat New Biol. 1971 Sep 29;233(39):131–134. doi: 10.1038/newbio233131a0. [DOI] [PubMed] [Google Scholar]
- Verma I. M., Temple G. F., Fan H., Baltimore D. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA. Nat New Biol. 1972 Feb 9;235(58):163–167. doi: 10.1038/newbio235163a0. [DOI] [PubMed] [Google Scholar]