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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Oct;70(10):2956–2959. doi: 10.1073/pnas.70.10.2956

Effects of 5-Bromo-2′-Deoxyuridine on Production of Globin Messenger RNA in Dimethyl Sulfoxide-Stimulated Friend Leukemia Cells

Harvey D Preisler *,, David Housman *,, William Scher *,, Charlotte Friend *,
PMCID: PMC427146  PMID: 4517948

Abstract

Friend leukemia cells grown in the presence of dimethyl sulfoxide show enhanced erythroid differentiation and hemoglobin synthesis. The effects of dimethyl sulfoxide stimulation are inhibited by BrdU. To determine the effect of BrdU on the amount of globin mRNA present in cells treated and not treated with dimethyl sulfoxide, molecular hybridization between total cell RNA and [3H]DNA complementary to mouse globin mRNA was used. Cells treated with BrdU and dimethyl sulfoxide had 70% less globin mRNA than cells treated with dimethyl sulfoxide alone. The size and base sequence of the residual globin mRNA in the cultures treated with BrdU and dimethyl sulfoxide were unaltered. Cells treated with BrdU alone contained slightly more globin mRNA than did the untreated controls, suggesting that BrdU may have a dual effect in transcription of messenger RNA.

Keywords: differentiation, Friend leukemia virus

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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