Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Oct;70(10):2960–2963. doi: 10.1073/pnas.70.10.2960

Direct Evidence for Messenger Activity of Influenza Virion RNA

W Siegert 1, G Bauer 1, P H Hofschneider 1
PMCID: PMC427147  PMID: 4355376

Abstract

In a cell-free system of Escherichia coli, RNA from influenza virus particles is translated into a polypeptide antigenically identical with the ribonucleoprotein and several more proteins, some of which correspond in size to viral structural components.

Keywords: protein synthesis in vitro, gel electrophoresis, radioimmune assay

Full text

PDF
2960

Images in this article

2962Image
on p.2962

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blair C. D., Duesberg P. H. Myxovirus ribonucleic acids. Annu Rev Microbiol. 1970;24:539–574. doi: 10.1146/annurev.mi.24.100170.002543. [DOI] [PubMed] [Google Scholar]
  2. Chow N. L., Simpson R. W. RNA-dependent RNA polymerase activity associated with virions and subviral particles of myxoviruses. Proc Natl Acad Sci U S A. 1971 Apr;68(4):752–756. doi: 10.1073/pnas.68.4.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gielkens A. L.J., Salden M. H.L., Bloemendal H., Konings R. N.H. Translation of oncogenic viral RNA and eukaryotic messenger RNA in the E. coli cell-free system. FEBS Lett. 1972 Dec 15;28(3):348–352. doi: 10.1016/0014-5793(72)80747-6. [DOI] [PubMed] [Google Scholar]
  4. Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Klenk H. D., Rott R., Becht H. On the structure of the influenza virus envelope. Virology. 1972 Mar;47(3):579–591. doi: 10.1016/0042-6822(72)90547-8. [DOI] [PubMed] [Google Scholar]
  6. Klenk H. D., Scholtissek C., Rott R. Inhibition of glycoprotein biosynthesis of influenza virus by D-glucosamine and 2-deoxy-D-glucose. Virology. 1972 Sep;49(3):723–734. doi: 10.1016/0042-6822(72)90529-6. [DOI] [PubMed] [Google Scholar]
  7. Konings R. N., Ward R., Francke B., Hofschneider P. H. Gene order of RNA bacteriophage M 12. Nature. 1970 May 16;226(5246):604–607. doi: 10.1038/226604a0. [DOI] [PubMed] [Google Scholar]
  8. Pons M. W. Studies on the replication of influenza virus RNA. Virology. 1972 Mar;47(3):823–832. doi: 10.1016/0042-6822(72)90574-0. [DOI] [PubMed] [Google Scholar]
  9. Pons M. W. The inhibition of influenza virus RNA synthesis by actinomycin D and cycloheximide. Virology. 1973 Jan;51(1):120–128. doi: 10.1016/0042-6822(73)90372-3. [DOI] [PubMed] [Google Scholar]
  10. Schild G. C., Winter W. D., Brand C. M. Serological diagnosis of human influenza infections by immunoprecipitin techniques. Bull World Health Organ. 1971;45(4):465–471. [PMC free article] [PubMed] [Google Scholar]
  11. Scholtissek C., Becht H., Rott R. Inhibition of influenza RNA polymerase by specific antiserum. Virology. 1971 Jan;43(1):137–143. doi: 10.1016/0042-6822(71)90231-5. [DOI] [PubMed] [Google Scholar]
  12. Scholtissek C., Rott R. Ribonucleic acid nucleotidyl transferase induced in chick fibroblasts after infection with an influenza virus. J Gen Virol. 1969 Jan;4(1):125–137. doi: 10.1099/0022-1317-4-1-125. [DOI] [PubMed] [Google Scholar]
  13. Siegert W., Hofschneider P. H. A direct approach to study the messenger properties of influenza-virion RNA. FEBS Lett. 1973 Aug 15;34(2):145–146. doi: 10.1016/0014-5793(73)80778-1. [DOI] [PubMed] [Google Scholar]
  14. Siegert W., Konings R. N., Bauer H., Hofschneider P. H. Translation of avian myeloblastosis virus RNA in a cell-free lysate of Escherichia coli. Proc Natl Acad Sci U S A. 1972 Apr;69(4):888–891. doi: 10.1073/pnas.69.4.888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Skehel J. J., Burke D. C. Ribonucleic acid synthesis in chick embryo cells infected with fowl-plague virus. J Virol. 1969 Apr;3(4):429–438. doi: 10.1128/jvi.3.4.429-438.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Skehel J. J. Polypeptide synthesis in influenza virus-infected cells. Virology. 1972 Jul;49(1):23–36. doi: 10.1016/s0042-6822(72)80004-7. [DOI] [PubMed] [Google Scholar]
  17. Stavis R. L., August J. T. The biochemistry of RNA bacteriophage replication. Annu Rev Biochem. 1970;39:527–560. doi: 10.1146/annurev.bi.39.070170.002523. [DOI] [PubMed] [Google Scholar]
  18. Ward R., Konings R. N., Hofschneider P. H. Coat protein repression of bacteriophage M12 RNA directed polysome formation. Eur J Biochem. 1970 Nov;17(1):106–115. doi: 10.1111/j.1432-1033.1970.tb01142.x. [DOI] [PubMed] [Google Scholar]
  19. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES