FIG 3 .

Sirtuin-modulating drugs impact virus protein expression. (A) HCMV early and late protein levels are reduced upon treatment with the sirtuin activators resveratrol (Res) and CAY10602. Cells were infected with HCMV (10 IU/cell) and treated with the indicated activator or DMSO at 2 hpi. Immediate early (IE1), early (pUL26), and late (pUL99-GFP) viral protein levels were assessed by Western blotting at 24 hpi, 48 hpi, and 72 hpi, respectively. β-Actin was used as a loading control. (B) HCMV late viral protein levels are increased upon treatment with the SIRT1 inhibitor EX-527. Cells were infected with HCMV (10 IU/cell) and treated with EX-527 (10 µM) or DMSO at 2 hpi. Viral protein levels were assessed as for panel A.