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. 1973 Oct;70(10):3002–3006. doi: 10.1073/pnas.70.10.3002

Characterization of RNA from Noninfectious Virions Produced by Sarcoma Positive-Leukemia Negative Transformed 3T3 Cells

Leo A Phillips 1, Vincent W Hollis Jr 1, Robert H Bassin 1, Peter J Fischinger 1
PMCID: PMC427156  PMID: 4355380

Abstract

RNA from noninfectious virions produced by two established clonal lines of sarcoma positive-leukemia negative (S+L-)-transformed 3T3 cells has been characterized. RNA from virions or nucleoids of S+L--(C243) cells consisted of three to four sizes: ±44 S (6%), 28 S (17%), 18 S (38%), and <18 S (39%). 28S virion RNA contained some virus-specific information demonstrable by RNA·DNA hybridization with a DNA probe derived from the RNA-directed DNA polymerase product of murine sarcoma-leukemia virus, while parallel studies indicated that 28S ribosomal RNA from ribosomal subunits of transformed and nontransformed 3T3 cells did not contain virus-specific information. In contrast to the S+L-(C243) virions, RNA from virions or nucleoids of S+L-(D56) cells consisted of five sizes: 80 S (6%), 68 S (8%), 56 S (5%), 28 S (28%), and <28 S (53%). Thermal dissociation studies suggested that this S+L- genome is comprised of 28S RNA subunits. From these studies we postulate that the 28S viral RNA is most probably the monomeric genome of S+L- virions.

Keywords: density gradient sedimentation, poly(A), polysomes, rate zonal sedimentation, ribosomes, RNA·DNA hybridization

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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