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. 1973 Nov;70(11):3067–3071. doi: 10.1073/pnas.70.11.3067

Integration of Deoxyribonucleic Acid Specific for Rous Sarcoma Virus after Infection of Permissive and Nonpermissive Hosts

Harold E Varmus *, Peter K Vogt †, J Michael Bishop *
PMCID: PMC427171  PMID: 4361674

Abstract

A relatively simple but stringent technique was developed to detect the integration of virus-specific DNA into the genomes of higher organisms. In both permissive (duck) and nonpermissive (mammalian) cells which normally contain no nucleotide sequences specific for Rous sarcoma virus, transformation by the virus results in the appearance of DNA specific for Rous sarcoma virus covalently integrated into strands of host-cell DNA containing reiterated sequences. Early after infection of mouse or duck cells by Rous sarcoma virus, unintegrated DNA specific for the virus can be demonstrated.

Keywords: RNA tumor viruses, reassociation kinetics, duck cells

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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