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. 1973 Nov;70(11):3094–3098. doi: 10.1073/pnas.70.11.3094

A Mammalian Cell Mutant with a Temperature-Sensitive Leucyl-Transfer RNA Synthetase

L H Thompson 1,*, J L Harkins 1, C P Stanners 1
PMCID: PMC427177  PMID: 4361675

Abstract

A cell mutant of the Chinese hamster ovary line, which is temperature sensitive for protein synthesis, is specifically defective in vivo in its ability to charge tRNA with leucine. Cytoplasmic extracts exhibited temperature-sensitive leucyl-tRNA synthetase activity. It is, therefore, highly likely that the mutant has a structural alteration in leucyl-tRNA synthetase. The low leakiness and low reversion rate of this mutant, combined with the specificity of the defect in its protein-synthesizing machinery, make it an appealing tool for investigating regulatory mechanisms in animal cells.

Keywords: somatic cell genetics, CHO cells, conditional lethality, inhibition of protein synthesis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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