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. 1973 Nov;70(11):3108–3110. doi: 10.1073/pnas.70.11.3108

Cs2SO4 Gradients Containing Both Hg2+ and Ag+ Effect the Complete Separation of Satellite Deoxyribonucleic Acids Having Identical Densities in Neutral CsCl Gradients

Dorothy M Skinner 1, Wanda G Beattie 1
PMCID: PMC427180  PMID: 4522292

Abstract

A combination of both Ag+ and Hg2+ in Cs2SO4 effects the complete separation of two DNAs having identical densities in CsCl. Satellite DNAs of hermit crab, Pagurus pollicaris, and lobster, Homarus americanus, have been isolated by this means.

Keywords: hermit crab, lobster, density gradient centrifugation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beattie W. G., Skinner D. M. The diversity of satellite DNAs of Crustacea. Biochim Biophys Acta. 1972 Oct 11;281(2):169–178. doi: 10.1016/0005-2787(72)90169-4. [DOI] [PubMed] [Google Scholar]
  2. Brunk C. F., Leick V. Rapid equilibrium isopycnic CsC1 gradients. Biochim Biophys Acta. 1969 Mar 18;179(1):136–144. doi: 10.1016/0005-2787(69)90129-4. [DOI] [PubMed] [Google Scholar]
  3. Corneo G., Ginelli E., Soave C., Bernardi G. Isolation and characterization of mouse and guinea pig satellite deoxyribonucleic acids. Biochemistry. 1968 Dec;7(12):4373–4379. doi: 10.1021/bi00852a033. [DOI] [PubMed] [Google Scholar]
  4. DAVIDSON N., WIDHOLM J., NANDI U. S., JENSEN R., OLIVERA B. M., WANG J. C. PREPARATION AND PROPERTIES OF NATIVE CRAB DAT. Proc Natl Acad Sci U S A. 1965 Jan;53:111–118. doi: 10.1073/pnas.53.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doerfler W., Hogness D. S. Separation of the strands of poly d-TG: AC in alkaline CsCl. J Mol Biol. 1965 Nov;14(1):237–240. doi: 10.1016/s0022-2836(65)80243-1. [DOI] [PubMed] [Google Scholar]
  6. Izatt R. M., Christensen J. J., Rytting J. H. Sites and thermodynamic quantities associated with proton and metal ion interaction with ribonucleic acid, deoxyribonucleic acid, and their constituent bases, nucleosides, and nucleotides. Chem Rev. 1971 Oct;71(5):439–481. doi: 10.1021/cr60273a002. [DOI] [PubMed] [Google Scholar]
  7. KATZ S. The reversible reaction of Hg (II) and double-stranded polynucleotides. A step-function theory and its significance. Biochim Biophys Acta. 1963 Feb 26;68:240–253. doi: 10.1016/0006-3002(63)90139-2. [DOI] [PubMed] [Google Scholar]
  8. SWARTZ M. N., TRAUTNER T. A., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies on nearest neighbor base sequences in deoxyribonucleic acids. J Biol Chem. 1962 Jun;237:1961–1967. [PubMed] [Google Scholar]
  9. Skinner D. M., Beattie W. G., Kerr M. S., Graham D. E. Satellite DNAs in crustacea: 2 different components with the same density in neutral CsCl gradients. Nature. 1970 Aug 22;227(5260):837–839. doi: 10.1038/227837a0. [DOI] [PubMed] [Google Scholar]
  10. Skinner D. M. SATELLITE DNA'S IN THE CRABS Gecarcinus lateralis AND Cancer pagurus. Proc Natl Acad Sci U S A. 1967 Jul;58(1):103–110. doi: 10.1073/pnas.58.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Skinner D. M., Triplett L. L. The selective loss of DNA satellites on deproteinization with phenol. Biochem Biophys Res Commun. 1967 Sep 27;28(6):892–897. doi: 10.1016/0006-291x(67)90062-9. [DOI] [PubMed] [Google Scholar]
  12. VINOGRAD J., HEARST J. E. Equilibrium sedimentation of macromolecules and viruses in a density gradient. Fortschr Chem Org Naturst. 1962;20:373–422. [PubMed] [Google Scholar]
  13. Wells R. D., Larson J. E. Buoyant density studies on natural and synthetic deoxyribonucleic acids in neutral and alkaline solutions. J Biol Chem. 1972 Jun 10;247(11):3405–3409. [PubMed] [Google Scholar]
  14. Wells R. D., Ohtsuka E., Khorana H. G. Studies on polynucleotides. L. Synthetic deoxyribopolynucleotides as templates for the DNA polymerase of Escherichia coli: a new double-stranded DNA-like polymer containing repeating dinucleotide sequences. J Mol Biol. 1965 Nov;14(1):221–237. doi: 10.1016/s0022-2836(65)80242-x. [DOI] [PubMed] [Google Scholar]

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