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. 1973 Nov;70(11):3125–3129. doi: 10.1073/pnas.70.11.3125

Cytogenetic Engineering In Vivo: Restoration of Biologic Complement Activity to C5-Deficient Mice by Intravenous Inoculation of Hybrid Cells

Nelson L Levy *, Ralph Snyderman *,†, Roger L Ladda ‡,§, Rose Lieberman
PMCID: PMC427184  PMID: 4131744

Abstract

Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5-deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5-Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic “repair” in mammals.

Keywords: mouse splenic macrophages, chicken erythrocytes, mouse kidney cells, genetic repair, MuB1 protein

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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