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. 1995 Jan 31;92(3):860–864. doi: 10.1073/pnas.92.3.860

Separation of human breast cancer cells from blood by differential dielectric affinity.

F F Becker 1, X B Wang 1, Y Huang 1, R Pethig 1, J Vykoukal 1, P R Gascoyne 1
PMCID: PMC42720  PMID: 7846067

Abstract

Electrorotation measurements were used to demonstrate that the dielectric properties of the metastatic human breast cancer cell line MDA231 were significantly different from those of erythrocytes and T lymphocytes. These dielectric differences were exploited to separate the cancer cells from normal blood cells by appropriately balancing the hydrodynamic and dielectrophoretic forces acting on the cells within a dielectric affinity column containing a microelectrode array. The operational criteria for successful particle separation in such a column are analyzed and our findings indicate that the dielectric affinity technique may prove useful in a wide variety of cell separation and characterization applications.

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Selected References

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