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. 1973 Nov;70(11):3260–3264. doi: 10.1073/pnas.70.11.3260

Application of Fingerprinting Techniques to Iodinated Nucleic Acids

Hugh D Robertson *, Elizabeth Dickson *, Peter Model *, Wolf Prensky †
PMCID: PMC427212  PMID: 4522301

Abstract

Several techniques of RNA and DNA finger-printing and determination of sequence have been applied to nucleic acids labeled with 125I. Fingerprints of human 5S RNA and bacteriophage f2 RNA resemble those of their noniodinated counterparts both in complexity and in specific pattern. Iodination as used here is thus a general labeling procedure, and appears principally to label cytidine residues. This iodination method shows little sensitivity to potential structure in single-stranded RNA molecules, yields stable oligonucleotide products in a reproducible manner, and does not change the specificity of several ribonucleases and deoxyribonucleases.

Keywords: iodine-125, ribonuclease, 5S RNA, messenger RNA, RNA chemical labeling

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