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. 1973 Dec;70(12 Pt 1-2):3334–3338. doi: 10.1073/pnas.70.12.3334

Studies on Purified Eel Acetylcholine Receptor and Anti-Acetylcholine Receptor Antibody

Jim Patrick 1,*, Jon Lindstrom 1, Bill Culp 1, Jerri Mcmillan 1
PMCID: PMC427231  PMID: 4128544

Abstract

Rabbit antiserum against purified Electrophorus electricus acetylcholine receptor is studied using an immunoprecipitin assay to measure either antibody titer or concentration of toxin-binding sites in solubilized receptor preparations. This antiserum, unlike control serum, blocks the electrophysiological response of the electroplax to carbamylcholine. Toxin (α-neurotoxin, Naja naja) and several cholinergic ligands produce partial inhibition of the reaction of antiserum with purified acetylcholine receptor. Evidence is presented that some of the toxin-binding sites on receptor, purified by affinity chromatography on toxin-agarose conjugates, are occluded by toxin. In addition, evidence is presented that antireceptor antiserum will cause precipitation of more toxin-binding sites present in an initial extract than in purified receptor preparations.

Keywords: electroplax, neurotoxin, affinity chromatography

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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