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. 1973 Dec;70(12 Pt 1-2):3376–3381. doi: 10.1073/pnas.70.12.3376

Anaerobic Transport in Escherichia coli Membrane Vesicles*

Wilhelmus N Konings †, H Ronald Kaback ‡,§
PMCID: PMC427240  PMID: 4587250

Abstract

Anaerobic β-galactoside transport in whole cells and membrane vesicles from E. coli ML 308-225 is coupled to the oxidation of α-glycerol-P or D-lactate with fumarate as an electron acceptor. Alternatively, anaerobic β-galactoside transport may be coupled to the oxidation of formate utilizing nitrate as electron acceptor. Both anaerobic electron-transfer systems are induced by growth of the organisms under appropriate conditions. Components of both systems are loosely bound to the membrane, necessitating the use of a modified procedure for vesicle preparation in order to demonstrate anaerobic transport in vitro. Addition of ATP or an ATP-generating system to vesicles prepared from anaerobically-grown cells or inclusion of ATP or the ATP-generating system during preparation of vesicles does not stimulate transport. The results support the conclusion that active transport under anaerobic conditions is coupled primarily to electron flow.

Keywords: β-galactosides, D-lactate dehydrogenase, α-glycerol phosphate dehydrogenase, fumarate reductase, formate dehydrogenase, nitrate reductase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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