Table 1.
Antibody Table
| Peptide/Protein Target | Antigen Sequence (if known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised in; Monoclonal or Polyclonal | Dilution Used |
|---|---|---|---|---|---|
| EGFR | NA | EGF Receptor (D38B1) XP rabbit mAb | Cell Signaling Technology, catalog #4267 | Rabbit, monoclonal | 1:1000 for WB, 1:200 for IF |
| pEGFR | NA | Phospho-EGF receptor (Tyr1068) antibody | Cell Signaling Technology, catalog number 2234 | Rabbit, polyclonal | 1:1000 for WB |
| HER2 | NA | HER2/ErbB2 (D8F12) XP rabbit mAb | Cell Signaling Technology, catalog number 2234 | Rabbit, monoclonal | 1:1000 for WB, 1:200 for IF |
| pHER2 | NA | Phospho-HER2/ErbB2 (Tyr877) antibody | Cell Signaling Technology, catalog number 2241 | Rabbit, polyclonal | 1:1000 for WB |
| PRL | NA | Prolactin(B-8), sc-271758 | Santa Cruz Biotechnology, Inc, catalog number sc-271758 | Mouse, monoclonal | 1:1000 for WB, 1:200 for IF |
| Erk1/2 | NA | p44/42 MAPK (Erl1/2) (137F5) rabbit mAb | Cell Signaling Technology, catalog number 4695 | Rabbit, monoclonal | 1:1000 for WB |
| pErk1/2 | NA | Phospho-p44/42 MAPK (Erl1/2) (Thr202/Tyr204) antibody | Cell Signaling Technology, catalog number 9101 | Rabbit, polyclonal | 1:1000 for WB, 1:200 for IF |
| Akt | NA | Akt(pan)(11E7) rabbit mAb | Cell Signaling Technology, catalog number 4685 | Rabbit, monoclonal | 1:1000 for WB |
| pAkt | NA | Phospho-Akt (Ser473) (D9E) XP rabbit mAb | Cell Signaling Technology, catalog number 4060 | Rabbit, monoclonal | 1:1000 for WB, 1:200 for IF |
| Actin | NA | Monoclonal antiactin antibody produced in mouse | Sigma-Aldrich, A4700 | Mouse, monoclonal | 1:5000 for WB |