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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Dec;70(12 Pt 1-2):3390–3394. doi: 10.1073/pnas.70.12.3390

Formation of Lipid-Bound Oligosaccharides Containing Mannose. Their Role in Glycoprotein Synthesis

Nicolas H Behrens 1, Hector Carminatti 1, Roberto J Staneloni 1, Luis F Leloir 1, Ana I Cantarella 1
PMCID: PMC427243  PMID: 4519632

Abstract

Incubation of GDP-[14C]mannose with liver microsomes and magnesium ions led to the formation of dolichol monophosphate mannose and of other acidlabile compounds that contain oligosaccharides. Formation of these compounds was enhanced by addition of an acceptor lipid in the same fractions of DEAE-cellulose chromatography where bound dolichol is found. Alkaline treatment of the oligosaccharides, obtained by acid methanolysis, followed by paper electrophoresis, gave rise to the formation of two positively charged substances believed to be formed by deacetylation of hexosamine residues. Incubation of the oligosaccharide-containing compounds with liver microsomes and manganese ions resulted in a transfer to endogenous protein. The role of dolichol derivatives in glycoprotein synthesis is discussed.

Keywords: dolichol, glycolipid, GDP-Man, liver microsomes

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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