Abstract
A partial amino-acid sequence of Bacillus subtilis 168 flagellin is presented. The region of unassigned sequence in this 304-residue polypeptide chain spans residues 158-173. Comparison of the 27-residue aminoterminal CNBr peptide of B. subtilis 168 flagellin with that derived from the flagellin of the serologically unrelated strain of B. subtilis, W23, shows only three conservative substitutions, whereas the 16-residue carboxyl-terminal peptides derived from these flagellins were identical. The comparison of the very limited sequence information available on the flagellins of Salmonella and Proteus with that on B. subtilis indicates homology between these proteins.
Keywords: Salmonella, Proteus, cyanogen bromide cleavage, tryptic digestion
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bustin M., Cole R. D. Bisection of a lysine-rich histone by N-bromosuccinimide. J Biol Chem. 1969 Oct 10;244(19):5291–5294. [PubMed] [Google Scholar]
- Chang J. Y., Brown D. M., Glazer A. N. Characterization of the subunits of the flagella of Proteus vulgaris. J Biol Chem. 1969 Oct 10;244(19):5196–5200. [PubMed] [Google Scholar]
- Davidson B. E. The alignment of cyanogen bromide fragments from the flagellin of Salmonella adelaide. Eur J Biochem. 1971 Feb;18(4):524–529. doi: 10.1111/j.1432-1033.1971.tb01272.x. [DOI] [PubMed] [Google Scholar]
- Emerson S. U., Simon M. I. Variation in the primary structure of Bacillus subtilis flagellins. J Bacteriol. 1971 Jun;106(3):949–954. doi: 10.1128/jb.106.3.949-954.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glossmann H., Bode W. Cyanogen bromide cleavage of Proteus mirabilis flagellin. Maleylation of cyanogen bromide peptides. Hoppe Seylers Z Physiol Chem. 1972 Mar;353(3):298–306. doi: 10.1515/bchm2.1972.353.1.298. [DOI] [PubMed] [Google Scholar]
- Iino T. Genetics and chemistry of bacterial flagella. Bacteriol Rev. 1969 Dec;33(4):454–475. doi: 10.1128/br.33.4.454-475.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joys T. M., Rankis V. The primary structure of the phase-1 flagellar protein of Salmonella typhimurium. I. The tryptic peptides. J Biol Chem. 1972 Aug 25;247(16):5180–5193. [PubMed] [Google Scholar]
- MARTINEZ R. J. A METHOD FOR THE PURIFICATION OF BACTERIAL FLAGELLA BY ION EXCHANGE CHROMATOGRAPHY. J Gen Microbiol. 1963 Oct;33:115–120. doi: 10.1099/00221287-33-1-115. [DOI] [PubMed] [Google Scholar]
- MCDONOUGH M. W. AMINO ACID COMPOSITION OF ANTIGENICALLY DISTINCT SALMONELLA FLAGELLAR PROTEINS. J Mol Biol. 1965 Jun;12:342–355. doi: 10.1016/s0022-2836(65)80258-3. [DOI] [PubMed] [Google Scholar]
- Martinez R. J., Brown D. M., Glazer A. N. The formation of bacterial flagella. 3. Characterization of the subunits of the flagella of Bacillus subtilis and Spirillum serpens. J Mol Biol. 1967 Aug 28;28(1):45–51. doi: 10.1016/s0022-2836(67)80076-7. [DOI] [PubMed] [Google Scholar]
- Martinez R. J., Gordee E. Z. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in spirillum serpens and bacillus subtilis. J Bacteriol. 1966 Feb;91(2):870–875. doi: 10.1128/jb.91.2.870-875.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martinez R. J., Ichiki A. T., Lundh N. P., Tronick S. R. A single amino acid substitution responsible for altered flagellar morphology. J Mol Biol. 1968 Jun 28;34(3):559–564. doi: 10.1016/0022-2836(68)90180-0. [DOI] [PubMed] [Google Scholar]
- Smith R. W., Koffler H. Bacterial flagella. Adv Microb Physiol. 1971;6:219–339. doi: 10.1016/s0065-2911(08)60070-3. [DOI] [PubMed] [Google Scholar]
- Yamaguchi S., Iino T. Serological and finger-printing analyses of mutant flagellar antigens of Salmonella. J Gen Microbiol. 1970 Dec;64(3):311–318. doi: 10.1099/00221287-64-3-311. [DOI] [PubMed] [Google Scholar]
