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. 1973 Dec;70(12 Pt 1-2):3450–3453. doi: 10.1073/pnas.70.12.3450

Inhibition of the Oxidation of Glutamate and Isocitrate in Liver Mitochondria at a Specific NADP+-Reducing Site

Diane C Lin 1,2, Ernest Kun 1,2
PMCID: PMC427257  PMID: 4148701

Abstract

The cation-complexing carboxylic-acid antibiotic X-537A, at concentrations far below that required for ionophorous activity, selectively inhibits the oxidation of glutamate and isocitrate by liver mitochondria in steady-state 3. The site of inhibition has been localized specifically at the reduction of NADP+. Glutamate and isocitrate dehydrogenases, the oxidation of NAD+-dependent substrates, pyridine nucleotide transhydrogenations, and the respiratory chain between NADH (or NADPH) and O2 are unaffected by the antibiotic X-537A. Kinetic evidence, i.e., competition between chlorotetracycline (a fluorescence probe for membrane-bound bivalent cation) and X-537A, indicates that the NADP+-reducing, antibiotic-sensitive site is most probably associated with the inner mitochondrial membrane.

Keywords: antibiotic X-537A, chlorotetracycline, membrane

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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