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. 1973 Dec;70(12 Pt 1-2):3454–3457. doi: 10.1073/pnas.70.12.3454

Regulation of Hepatic Glycogen Synthesis During Fetal Development: Roles of Hydrocortisone, Insulin, and Insulin Receptors

Howard J Eisen *,‡, Ira D Goldfine *, Walter H Glinsmann *,‡
PMCID: PMC427258  PMID: 4357871

Abstract

In fetal rat liver in utero, an increase in glycogen and the glycogen synthetic enzyme glycogen synthetase (EC 2.4.1.11) occurs between gestational days 17 and 19. We used an organ culture system for finding the stimulus for increased enzyme activity and defining the relationship of this increase to glycogen synthesis. In fetal-liver explants from an earlier period (gestational day 16), hydrocortisone causes an elevation in total glycogen synthetase activity. This effect, which can be blocked by actinomycin D, is strikingly similar in time course and magnitude to the normal increase in utero. However, in order for glycogen synthesis to proceed after hydrocortisone increases glycogen synthetase levels, insulin is required. Unlike hydrocortisone, insulin does not increase total glycogen synthetase; it appears to act by converting the b or phospho form of synthetase to the a or dephospho form. Insulin alone does not stimulate glycogen synthesis in explants from 16-day fetal liver, although no defect in insulin binding was demonstrable. These findings support the hypothesis that the increase in liver-glycogen synthesis during the last trimester requires glucocorticoids for the developmental induction of glycogen synthetase and insulin for activation of the enzyme.

Keywords: glycogen synthetase, organ culture, glucocorticoids

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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