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. 1973 Dec;70(12 Pt 1-2):3498–3501. doi: 10.1073/pnas.70.12.3498

Hybridization of Leucyl-Transfer Ribonucleic Acid Isoacceptors from Green Leaves with Nuclear and Chloroplast Deoxyribonucleic Acid

Gene R Williams 1, Alice S Williams 1, S A George 1
PMCID: PMC427267  PMID: 16592122

Abstract

Chromatographically distinct isoacceptors of leucyl-tRNA from mature bean leaves were all observed to hybridize with bean chloroplast and nuclear DNA in a ratio similar to that exhibited by the unfractionated leucyl-tRNA. Under the same hybridization conditions, maize tRNA failed to form a stable hybrid with bean DNA, and levels of hybridization between bean-leaf leucyl-tRNA and nuclear or chloroplast DNAs from tobacco and maize were relatively small.

Keywords: beans, tobacco, maize

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARNETT W. E., JACOBSON K. B. EVIDENCE FOR DEGENERACY AND AMBIGUITY IN INTERSPECIES AMINOACYL-SRNA FORMATION. Proc Natl Acad Sci U S A. 1964 Apr;51:642–647. doi: 10.1073/pnas.51.4.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnett W. E., Brown D. H., Epler J. L. Mitochondrial-specific aminoacyl-RNA synthetases. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1775–1781. doi: 10.1073/pnas.57.6.1775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buck C. A., Nass M. M. Differences between mitochondrial and cytoplasmic transfer RNA and aminoacyl transfer RNA synthetases from rat liver. Proc Natl Acad Sci U S A. 1968 Jul;60(3):1045–1052. doi: 10.1073/pnas.60.3.1045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  5. KIRBY K. S. ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID. Biochem J. 1965 Jul;96:266–269. doi: 10.1042/bj0960266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MARCUS A. Amino acid dependent exchange between pyrophosphate and adenosine triphosphate in spinach preparations. J Biol Chem. 1959 May;234(5):1238–1240. [PubMed] [Google Scholar]
  7. Reger B. J., Fairfield S. A., Epler J. L., Barnett W. E. Identification and origin of some chloroplast aminoacyl-tRNA synthetases and tRNAs. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1207–1213. doi: 10.1073/pnas.67.3.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SISSAKIAN N. M., FILIPPOVICH I. I., SVETAILO E. N., ALIYEV K. A. ON THE PROTEIN-SYNTHESIZING SYSTEM OF CHLOROPLASTS. Biochim Biophys Acta. 1965 Mar 15;95:474–485. doi: 10.1016/0005-2787(65)90193-0. [DOI] [PubMed] [Google Scholar]
  9. Suyama Y., Eyer J. Leucyl tRNA and leucyl tRNA synthetase in mitochondria of Tetrahymena pyriformis. Biochem Biophys Res Commun. 1967 Sep 7;28(5):746–751. doi: 10.1016/0006-291x(67)90379-8. [DOI] [PubMed] [Google Scholar]
  10. Tewari K. K., Wildman S. G. Function of chloroplast DNA. I. Hybridization studies involving nuclear and chloroplast DNA with RNA from cytoplasmic (80S) and chloroplast (70S) ribosomes. Proc Natl Acad Sci U S A. 1968 Feb;59(2):569–576. doi: 10.1073/pnas.59.2.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weiss J. F., Kelmers A. D. A new chromatographic system for increased resolution of transfer ribonucleic acids. Biochemistry. 1967 Aug;6(8):2507–2513. doi: 10.1021/bi00860a030. [DOI] [PubMed] [Google Scholar]
  12. Weiss S. B., Hsu W. T., Foft J. W., Scherberg N. H. Transfer RNA coded by the T4 bacteriophage genome. Proc Natl Acad Sci U S A. 1968 Sep;61(1):114–121. doi: 10.1073/pnas.61.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Whitfeld P. R., Spencer D. Buoyant density of tobacco and spinach chloroplast DNA. Biochim Biophys Acta. 1968 Apr 22;157(2):333–343. doi: 10.1016/0005-2787(68)90087-7. [DOI] [PubMed] [Google Scholar]
  14. Williams G. R., Williams A. S. Hybridization of bean leaf leucyl-tRNA with nuclear DNA and with chloroplast DNA. Biochem Biophys Res Commun. 1970 Jun 5;39(5):858–863. doi: 10.1016/0006-291x(70)90402-x. [DOI] [PubMed] [Google Scholar]

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