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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 Dec;70(12 Pt 1-2):3566–3570. doi: 10.1073/pnas.70.12.3566

In Vitro Synthesis of RNA That Contains Polyadenylate by Virion-Associated RNA Polymerase of Vesicular Stomatitis Virus

Amiya K Banerjee 1, Dennis P Rhodes 1
PMCID: PMC427281  PMID: 4357880

Abstract

The RNA synthesized in vitro by the virion-associated RNA-instructed RNA polymerase of purified vesicular stomatitis virus contains polyadenylate sequences. These have been demonstrated by their partial resistance to pancreatic and T1 ribonucleases and their capacity to bind to poly(U) filters and oligo(dT)-cellulose. The polyadenylate sequences range in apparent size from 50 to 200 bases, similar to the size of the poly(A) in mRNA from vesicular stomatitis virus-infected cells. Possible mechanisms of polyadenylylation of the in vitro RNA product are discussed.

Keywords: poly(U) filters, oligo(dT)-cellulose, RNA gel electrophoresis, transcriptase, viral replication

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Banerjee A. K., Shatkin A. J. Transcription in vitro by reovirus-associated ribonucleic acid-dependent polymerase. J Virol. 1970 Jul;6(1):1–11. doi: 10.1128/jvi.6.1.1-11.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop D. H. Complete transcription by the transcriptase of vesicular stomatitis virus. J Virol. 1971 Apr;7(4):486–490. doi: 10.1128/jvi.7.4.486-490.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bishop D. H., Roy P. Kinetics of RNA synthesis by vesicular stomatitis virus particles. J Mol Biol. 1971 May 14;57(3):513–527. doi: 10.1016/0022-2836(71)90106-9. [DOI] [PubMed] [Google Scholar]
  5. Bishop D. H., Roy P. Properties of the product synthesized by vesicular stomatitis virus particles. J Mol Biol. 1971 Jun 28;58(3):799–814. doi: 10.1016/0022-2836(71)90041-6. [DOI] [PubMed] [Google Scholar]
  6. CHAMBERLIN M., BERG P. MECHANISM OF RNA POLYMERASE ACTION: CHARACTERIZATION OF THE DNA-DEPENDENT SYNTHESIS OF POLYADENYLIC ACID. J Mol Biol. 1964 May;8:708–726. doi: 10.1016/s0022-2836(64)80120-0. [DOI] [PubMed] [Google Scholar]
  7. Darnell J. E., Philipson L., Wall R., Adesnik M. Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA. Science. 1971 Oct 29;174(4008):507–510. doi: 10.1126/science.174.4008.507. [DOI] [PubMed] [Google Scholar]
  8. EDMONDS M., ABRAMS R. Polynucleotide biosynthesis: formation of a sequence of adenylate units from adenosine triphosphate by an enzyme from thymus nuclei. J Biol Chem. 1960 Apr;235:1142–1149. [PubMed] [Google Scholar]
  9. Ehrenfeld E., Summers D. F. Adenylate-rich sequences in vesicular stomatitis virus messenger ribonucleic acid. J Virol. 1972 Oct;10(4):683–688. doi: 10.1128/jvi.10.4.683-688.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Galet H., Prevec L. Polyadenylate synthesis by extracts from L cells infected with vesicular stomatitis virus. Nat New Biol. 1973 Jun 13;243(128):200–203. doi: 10.1038/newbio243200a0. [DOI] [PubMed] [Google Scholar]
  11. Huang A. S., Baltimore D., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus. 3. Multiple complementary messenger RNA molecules. Virology. 1970 Dec;42(4):946–957. doi: 10.1016/0042-6822(70)90343-0. [DOI] [PubMed] [Google Scholar]
  12. Huang A. S., Greenawalt J. W., Wagner R. R. Defective T particles of vesicular stomatitis virus. I. Preparation, morphology, and some biologic properties. Virology. 1966 Oct;30(2):161–172. doi: 10.1016/0042-6822(66)90092-4. [DOI] [PubMed] [Google Scholar]
  13. Huang A. S., Manders E. K. Ribonucleic acid synthesis of vesicular stomatitis virus. IV. Transcription by standard virus in the presence of defective interfering particles. J Virol. 1972 Jun;9(6):909–916. doi: 10.1128/jvi.9.6.909-916.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Huang A. S., Wagner R. R. Comparative sedimentation coefficients of RNA extracted from plaque-forming and defective particles of vesicular stomatitis virus. J Mol Biol. 1966 Dec 28;22(2):381–384. doi: 10.1016/0022-2836(66)90143-4. [DOI] [PubMed] [Google Scholar]
  15. Marshall S., Gillespie D. Poly U tracts absent from viral RNA. Nat New Biol. 1972 Nov 8;240(97):43–45. doi: 10.1038/newbio240043a0. [DOI] [PubMed] [Google Scholar]
  16. Molloy G. R., Thomas W. L., Darnell J. E. Occurrence of uridylate-rich oligonucleotide regions in heterogeneous nuclear RNA of HeLa cells. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3684–3688. doi: 10.1073/pnas.69.12.3684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mudd J. A., Summers D. F. Polysomal ribonucleic acid of vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Dec;42(4):958–968. doi: 10.1016/0042-6822(70)90344-2. [DOI] [PubMed] [Google Scholar]
  18. Philipson L., Wall R., Glickman G., Darnell J. E. Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806–2809. doi: 10.1073/pnas.68.11.2806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Scherrer K., Marcaud L., Zajdela F., Breckenridge B., Gros F. Etude des RNA nucléaires et cytoplasmiques à marquage rapide dans les cellules érythropoiétiques aviaires différenciées. Bull Soc Chim Biol (Paris) 1966;48(10):1037–1075. [PubMed] [Google Scholar]
  20. Schincariol A. L., Howatson A. F. Replication of vesicular stomatitis virus. I. Viral specific RNA and nucleoprotein in infected L cells. Virology. 1970 Nov;42(3):732–743. doi: 10.1016/0042-6822(70)90319-3. [DOI] [PubMed] [Google Scholar]
  21. Schincariol A. L., Howatson A. F. Replication of vesicular stomatitis virus. II. Separation and characterization of virus-specific RNA species. Virology. 1972 Sep;49(3):766–783. doi: 10.1016/0042-6822(72)90533-8. [DOI] [PubMed] [Google Scholar]
  22. Sheldon R., Jurale C., Kates J. Detection of polyadenylic acid sequences in viral and eukaryotic RNA(polu(U)-cellulose columns-poly(U) filters-fiberglass-HeLa cells-bacteriophage T4). Proc Natl Acad Sci U S A. 1972 Feb;69(2):417–421. doi: 10.1073/pnas.69.2.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wagner R. R., Prevec L., Brown F., Summers D. F., Sokol F., MacLeod R. Classification of rhabdovirus proteins: a proposal. J Virol. 1972 Dec;10(6):1228–1230. doi: 10.1128/jvi.10.6.1228-1230.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weinberg R. A., Ben-Ishai Z., Newbold J. E. Poly A associated with SV40 messenger RNA. Nat New Biol. 1972 Jul 26;238(82):111–113. doi: 10.1038/newbio238111a0. [DOI] [PubMed] [Google Scholar]
  25. Wild T. F. Replication of vesicular stomatitis virus: characterization of the virus-induced RNA. J Gen Virol. 1971 Nov;13(2):295–310. doi: 10.1099/0022-1317-13-2-295. [DOI] [PubMed] [Google Scholar]

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